Lactobacilli express cell surface proteins which mediate binding of immobilized collagen and fibronectin

被引:5
作者
Lorca, G
Torino, MI
de Valdez, GF
Ljungh, Å
机构
[1] Consejo Nacl Invest Cient & Tecn, CERELA, Ctr Referencia Labtobacilos, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[2] Lund Univ, Dept Med Microbiol Dermatol & Infect, S-22362 Lund, Sweden
关键词
Lactobacillus; collagen binding; fibronectin binding; polysaccharide; immunoblot; protein-protein interaction;
D O I
10.1016/S0378-1097(01)00512-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Binding of immobilized collagen-I (Cn-I) and fibronectin (Fn) by Lactobacillus acidophilus CRL 639 depends on cell-surface proteins. Capsule formation during the stationary growth phase has a negative effect on adherence of Cn-I and Fn. However, cells from the exponential growth phase. which produce no capsule, exhibit maximal binding. Binding is sensitive to trypsin, proteinase K, pronase E, and heat. Gelatin and soluble Cn-I partially inhibit binding of Cn-I although various proteins, sugars and amino acids do not affect binding to Fn. These results indicate that protein-protein interactions mediate adhesion to extracellular matrix proteins. SDS-PAGE and Western blot analyses of surface proteins revealed that several proteins including the major 43-kDa protein of the S-layer are expressed. Monoclonal antibodies showed that Fn binds to a 15-kDa protein, while Cn-I binds to proteins of 45 and 58 kDa. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
引用
收藏
页码:31 / 37
页数:7
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