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Conversion from CUL4-based COP1-SPA E3 apparatus to UVR8-COP1-SPA complexes underlies a distinct biochemical function of COP1 under UV-B

被引：159
作者：
Huang, Xi [1 ,2 ]
Ouyang, Xinhao [1 ,2 ,3 ]
Yang, Panyu [1 ,4 ]
Lau, On Sun [2 ]
Chen, Liangbi [4 ]
Wei, Ning [2 ]
Deng, Xing Wang [1 ,2 ]
机构：
[1] Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, Beijing 100871, Peoples R China
[2] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[3] Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Sichuan, Peoples R China
[4] Hunan Normal Univ, Coll Life Sci, Dept Bot, Changsha 410081, Hunan, Peoples R China
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 41期
基金：
中国博士后科学基金;
关键词：
light signaling; protein complex; posttranscriptional regulation; CONSTITUTIVELY PHOTOMORPHOGENIC1; LIGHT CONTROL; ARABIDOPSIS; PROTEINS; HY5; TRANSCRIPTION; SUPPRESSION; PERCEPTION; INTERACTS; GROWTH;
D O I：
10.1073/pnas.1316622110
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The evolutionarily conserved CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) is a RING and WD40 protein that functions as a substrate receptor of CULLIN4-DAMAGED DNA BINDING PROTEIN 1 (CUL4-DDB1)-based E3 ubiquitin ligases in both plants and animals. In Arabidopsis, COP1 is a central repressor of photomorphogenesis in the form of COP1-SUPPRESSOR OF PHYA (SPA) complex(es). CUL4-DDB1-COP1-SPA suppresses the photomorphogenic program by targeting the transcription factor ELONGATED HYPOCOTYL 5 for degradation. Intriguingly, under photomorphogenic UV-B light, COP1 reverses its repressive role and promotes photomorphogenesis. However, the mechanism by which COP1 is functionally switched is still obscure. Here, we demonstrate that UV-B triggers the physical and functional disassociation of the COP1-SPA core complex(es) from CUL4-DDB1 and the formation of a unique complex(es) containing the UV-B receptor UV RESISTANCE LOCUS 8 (UVR8). The establishment of this UV-B-dependent COP1 complex(es) is associated with its positive modulation of ELONGATED HYPOCOTYL 5 stability and activity, which sheds light on the mechanism of COP1's promotive action in UV-B-induced photomorphogenesis.
引用
收藏
页码：16669 / 16674
页数：6
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