Determination of histamine by capillary zone electrophoresis using a low pH phosphate buffer: Application in the analysis of fish and marine products

Histamine levels in samples of fish and marine products were determined by capillary zone electrophoresis using phosphate buffer at pH 2.44 and UV detection at 214 nm. A plot of the area of the peaks versus histamine concentration was linear over the range of 1-100 ppm with a correlation coefficient of 0.9996. Calibration using the height of the peak also gave good correlation, with a correlation coefficient of 0.9969, at concentrations between 1 ppm and 20 ppm. The recovery of added histamine in different samples of fish and fish products was, on average, 99.65%. The method described in this work was used for the quantitation of histamine in fish and fish products.