Electrochemical Nanoprobes for Single-Cell Analysis

被引：203

作者：

Actis, Paolo ^{[1
]}

Tokar, Sergiy ^{[1
]}

Clausmeyer, Jan ^{[2
]}

Babakinejad, Babak ^{[1
]}

Mikhaleva, Sofya ^{[1
]}

Cornut, Renaud ^{[4
]}

Takahashi, Yasufumi ^{[3
]}

Cordoba, Ainara Lopez ^{[1
]}

Novak, Pavel ^{[5
]}

Shevchuck, Andrew I. ^{[1
]}

Dougan, Jennifer A. ^{[6
]}

Kazarian, Sergei G. ^{[6
]}

Gorelkin, Petr V. ^{[7
]}

Erofeev, Alexander S. ^{[7
]}

Yaminsky, Igor V. ^{[7
]}

Unwin, Patrick R. ^{[8
]}

Schuhmann, Wolfgang ^{[2
]}

Klenerman, David ^{[9
]}

Rusakov, Dmitri A. ^{[10
]}

Sviderskaya, Elena V. ^{[11
]}

Korchev, Yuri E. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Med, London W12 0NN, England

[2] Ruhr Univ Bochum, D-44801 Bochum, Germany

[3] Tohoku Univ, Adv Inst Mat Res, Sendai, Miyagi 9808577, Japan

[4] CEA Saclay, Lab Chim Surfaces & Interfaces, F-91191 Gif Sur Yvette, France

[5] Univ London, Sch Engn & Mat Sci, London E1 4NS, England

[6] Univ London Imperial Coll Sci Technol & Med, Dept Chem Engn, London SW7 2AZ, England

[7] Med Nanotechnol, Moscow 119311, Russia

[8] Univ Warwick, Dept Chem, Coventry CV4 7AL, W Midlands, England

[9] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

[10] UCL, UCL Inst Neurol, London WC1N 3BG, England

[11] St Georges Univ London, Div Biomed Sci, London SW17 0RE, England

来源：

ACS NANO | 2014年 / 8卷 / 01期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会; 欧洲研究理事会;

关键词：

carbon; nanoelectrode; platinum; electrochemistry; intracellular measurements; brain slice; melanoma; HYDROGEN-PEROXIDE; REACTIVE OXYGEN; MICROSCOPY; ELECTRODES; PROBE; MICROELECTRODES; SUBMICROMETER; REDUCTION; DELIVERY; CURRENTS;

D O I：

10.1021/nn405612q

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microscopy probes, which should allow high resolution electrochemical mapping of species on or in living cells.

引用

页码：875 / 884

页数：10

共 57 条

[1]

Compartmental Genomics in Living Cells Revealed by Single-Cell Nanobiopsy [J].

Actis, Paolo ;

Maalouf, Michelle M. ;

Kim, Hyunsung John ;

Lohith, Akshar ;

Vilozny, Boaz ;

Seger, R. Adam ;

Pourmand, Nader .

ACS NANO, 2014, 8 (01) :546-553

[2]

Actis Paolo, 2010, Bioanal Rev, V1, P177

[3]

Monitoring in real time with a microelectrode the release of reactive oxygen and nitrogen species by a single macrophage stimulated by its membrane mechanical depolarization [J].

Amatore, C ;

Arbault, S ;

Bouton, C ;

Coffi, K ;

Drapier, JC ;

Ghandour, H ;

Tong, YH .

CHEMBIOCHEM, 2006, 7 (04) :653-661

[4]

Neuronal Recordings with Solid-Conductor Intracellular Nanoelectrodes (SCINEs) [J].

Angle, Matthew R. ;

Schaefer, Andreas T. .

PLOS ONE, 2012, 7 (08)

[5]

Monitoring extracellular pH, oxygen, and dopamine during reward delivery in the striatum of primates [J].

Ariansen, Jennifer L. ;

Heien, Michael L. A. V. ;

Hermans, Andre ;

Phillips, Paul E. M. ;

Hernadi, Istvan ;

Bermudez, Maria A. ;

Schultz, Wolfram ;

Wightman, R. Mark .

FRONTIERS IN BEHAVIORAL NEUROSCIENCE, 2012, 6

[6]

Local Delivery of Molecules from a Nanopipette for Quantitative Receptor Mapping on Live Cells [J].

Babakinejad, Babak ;

Joensson, Peter ;

Lopez Cordoba, Ainara ;

Actis, Paolo ;

Novak, Pavel ;

Takahashi, Yasufumi ;

Shevchuk, Andrew ;

Anand, Uma ;

Anand, Praveen ;

Drews, Anna ;

Ferrer-Montiel, Antonio ;

Klenerman, David ;

Korchev, Yuri E. .

ANALYTICAL CHEMISTRY, 2013, 85 (19) :9333-9342

[7]

Chemically modified microelectrodes designed for the electrochemical determination of nitric oxide in biological systems [J].

Bedioui, F ;

Trevin, S ;

Devynck, J .

ELECTROANALYSIS, 1996, 8 (12) :1085-1091

[8]

Small Molecule Injection into Single-Cell C. elegans Embryos via Carbon-Reinforced Nanopipettes [J].

Brennan, Lucy D. ;

Roland, Thibault ;

Morton, Diane G. ;

Fellman, Shanna M. ;

Chung, SueYeon ;

Soltani, Mohammad ;

Kevek, Joshua W. ;

McEuen, Paul M. ;

Kemphues, Kenneth J. ;

Wang, Michelle D. .

PLOS ONE, 2013, 8 (09)

[9]

Microelectrodes for the measurement of catecholamines in biological systems [J].

Cahill, PS ;

Walker, QD ;

Finnegan, JM ;

Mickelson, GE ;

Travis, ER ;

Wightman, RM .

ANALYTICAL CHEMISTRY, 1996, 68 (18) :3180-3186

[10]

Scanning Ion Conductance Microscopy [J].

Chen, Chiao-Chen ;

Zhou, Yi ;

Baker, Lane A. .

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 5, 2012, 5 :207-228

← 1 2 3 4 5 6 →