Purification and properties of the raw starch-degrading α-amylase of Bacillus sp. IMD 434

被引：53

作者：

Hamilton, LM ^{[1
]}

Kelly, CT ^{[1
]}

Fogarty, WM ^{[1
]}

机构：

[1] Natl Univ Ireland Univ Coll Dublin, Dept Ind Microbiol, Dublin 4, Ireland

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 02期

关键词：

Bacillus; alpha-amylase; purification; raw starch-degrading;

D O I：

10.1023/A:1005413816101

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The paw starch-degrading alpha-amylase of Bacillus sp. IMD 434 was purified to homogeneity by acetone precipitation, ion-exchange chromatography and hydrophobic interaction chromatography. The enzyme had a relative molecular mass of 69,200, displayed maximum activity at pH 6.0 and 65 degrees C and released large amounts of glucose and maltose an hydrolysis of starch.

引用

页码：111 / 115

页数：5

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