A new isolation procedure and further characterisation of the lectin from the red marine alga Ptilota serrata

被引：20

作者：

Sampaio, AH

Rogers, DJ

Barwell, CJ

Saker-Sampaio, S

Costa, FHF

Ramos, MV

机构：

[1] Fed Univ Ceara, BioMol Lab, Dept Fishing Engn, BR-60451970 Fortaleza, Ceara, Brazil

[2] Univ Portsmouth, Sch Pharm & Biomed Sci, Portsmouth PO1 2DT, Hants, England

[3] Fed Univ Ceara, BioMol Lab, Dept Biol, Fortaleza, Ceara, Brazil

来源：

JOURNAL OF APPLIED PHYCOLOGY | 1998年 / 10卷 / 06期

关键词：

Ptilota serrata; red marine alga; lectin; agglutinin; affinity chromatography; guar gum; sugar inhibition;

D O I：

10.1023/A:1008061327247

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ptilota serrata has been shown previously to contain a lectin (PSL) which is non-specific for human blood groups. We report here a new isolation procedure for PSL using a single step affinity chromatography technique on cross-linked guar gum and further characterisation studies. PSL was inhibited by galactose and its derivatives. The carbohydrates o-nitrophenyl-N-acetyl-alpha-D-galactoside, p-nitrophenyl-N-acetyl-B-D-galactoside and lactose were strong inhibitors. The glycoproteins porcine stomach mucin, asialo bovine mucin and asialofetuin were also inhibitory. The Mr of PSL, determined by gel filtration, was 55,470. SDS-PAGE revealed one single protein band with Mr of 18,390, indicating the native protein to be a trimer of apparently identical subunits. PSL was shown to contain large amounts of acidic and hydroxyl amino acids but low in basic amino acids.

引用

页码：539 / 546

页数：8