A novel gene, DXS8237E. lies within 20 kb upstream of UBE1 in Xp11.23 and has a different X inactivation status

被引：24

作者：

Coleman, MP

Ambrose, HJ

Carrel, L

Nemeth, AH

Willard, HF

Davies, KE

机构：

[1] JOHN RADCLIFFE HOSP,INST MOLEC MED,MOLEC GENET GRP,OXFORD OX3 9LU,ENGLAND

[2] CASE WESTERN RESERVE UNIV,SCH MED,DEPT GENET,CLEVELAND,OH 44106

[3] CASE WESTERN RESERVE UNIV,SCH MED,CTR HUMAN GENET,CLEVELAND,OH 44106

来源：

GENOMICS | 1996年 / 31卷 / 01期

基金：

美国国家卫生研究院; 英国医学研究理事会;

关键词：

D O I：

10.1006/geno.1996.0022

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel X-linked gene, DXS8237E, was isolated from human fetal brain cDNA, and its 3' end was mapped to within 20 kb upstream of UBE1 in Xp11.23. A 1.3-kb cDNA for DXS8237E detects homologous sequences in other mammals and a 3-kb mRNA that is widely expressed in human cell lines and mouse tissues. Sequence analysis indicated that the 1.3-kb cDNA contains the 3' end of the DXS8237E gene, but the sequence shows no significant homology to known genes. DXS8237E was shown to be subject to X inactivation in five somatic cell hybrids that contain an inactive human X chromosome but no active homologue. Since UBE1 escapes X inactivation, DXS8237E and UBE1 are the closest mapped genes with discordant X inactivation profiles. Sequences in the vicinity of these two genes may be important determinants of X inactivation status. (C) 1996 Academic Press, Inc.

引用

页码：135 / 138

页数：4

共 18 条

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