4D chromatin dynamics in cycling cells Theodor Boveri's hypotheses revisited

被引:83
作者
Strickfaden, Hilmar [1 ]
Zunhammer, Andreas [1 ]
van Koningsbruggen, Silvana [2 ]
Koehler, Daniela [1 ]
Cremer, Thomas [1 ,3 ]
机构
[1] LMU Biozentrum, Dept Biol Anthropol & Human Genet 2, Martinsried, Germany
[2] Univ Dundee, Wellcome Trust Ctr Gene Regulat & Express, Coll Life Sci, Dundee, Scotland
[3] Munich Ctr Integrated Prot Sci Munich CIPSM, Munich, Germany
关键词
chromatin dynamics; 4D live-cell microscopy; Theodor Boveri; mitosis; nuclear rotation; long range chromatin movements; NUCLEAR ARCHITECTURE; CHROMOSOME TERRITORIES; INTERPHASE NUCLEI; HISTORICAL-PERSPECTIVE; SPATIAL-ORGANIZATION; GENOME; MOTION; ORDER; MITOSIS; LOCALIZATION;
D O I
10.4161/nucl.1.3.11969
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
This live cell study of chromatin dynamics in four dimensions (space and time) in cycling human cells provides direct evidence for three hypotheses first proposed by Theodor Boveri in seminal studies of fixed blastomeres from Parascaris equorum embryos: (I) Chromosome territory (CT) arrangements are stably maintained during interphase. (II) Chromosome proximity patterns change profoundly during prometaphase. (III) Similar CT proximity patterns in pairs of daughter nuclei reflect symmetrical chromosomal movements during anaphase and telophase, but differ substantially from the arrangement in mother cell nucleus. Hypothesis I could be confirmed for the majority of interphase cells. A minority, however, showed complex, rotational movements of CT assemblies with large-scale changes of CT proximity patterns, while radial nuclear arrangements were maintained. A new model of chromatin dynamics is proposed. It suggests that long-range DNA-DNA interactions in cell nuclei may depend on a combination of rotational CT movements and locally constrained chromatin movements.
引用
收藏
页码:284 / 297
页数:14
相关论文
共 84 条
[61]  
Rabl C., 1885, MORPHOL JB, V10, P214
[62]   A genetic analysis of chromosome territory looping: diverse roles for distal regulatory elements [J].
Ragoczy, T ;
Telling, A ;
Sawado, T ;
Groudine, M ;
Kosak, ST .
CHROMOSOME RESEARCH, 2003, 11 (05) :513-525
[63]   Macromolecular crowding and its potential impact on nuclear function [J].
Richter, Karsten ;
Nessling, Michelle ;
Lichter, Peter .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2008, 1783 (11) :2100-2107
[64]   Revealing the high-resolution three-dimensional network of chromatin and interchromatin space: A novel electron-microscopic approach to reconstructing nuclear architecture [J].
Rouquette, Jacques ;
Genoud, Christel ;
Vazquez-Nin, Gerardo H. ;
Kraus, Bernd ;
Cremer, Thomas ;
Fakan, Stanislav .
CHROMOSOME RESEARCH, 2009, 17 (06) :801-810
[65]   Embedding 3D Models of Biological Specimens in PDF Publications [J].
Ruthensteiner, Bernhard ;
Hess, Martin .
MICROSCOPY RESEARCH AND TECHNIQUE, 2008, 71 (11) :778-786
[66]   Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells [J].
Schoenfelder, Stefan ;
Sexton, Tom ;
Chakalova, Lyubomira ;
Cope, Nathan F. ;
Horton, Alice ;
Andrews, Simon ;
Kurukuti, Sreenivasulu ;
Mitchell, Jennifer A. ;
Umlauf, David ;
Dimitrova, Daniela S. ;
Eskiw, Christopher H. ;
Luo, Yanquan ;
Wei, Chia-Lin ;
Ruan, Yijun ;
Bieker, James J. ;
Fraser, Peter .
NATURE GENETICS, 2010, 42 (01) :53-U71
[67]   Genomic interactions: Chromatin loops and gene meeting points in transcriptional regulation [J].
Sexton, Tom ;
Bantignies, Frederic ;
Cavalli, Giacomo .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2009, 20 (07) :849-855
[68]   FISH-eyed and genome-wide views on the spatial organisation of gene expression [J].
Simonis, Marieke ;
de Laat, Wouter .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2008, 1783 (11) :2052-2060
[69]   Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C) [J].
Simonis, Marieke ;
Klous, Petra ;
Splinter, Erik ;
Moshkin, Yuri ;
Willemsen, Rob ;
de Wit, Elzo ;
van Steensel, Bas ;
de Laat, Wouter .
NATURE GENETICS, 2006, 38 (11) :1348-1354
[70]  
Solovei I, 2004, CHROMOSOMA, V112, P410