Bilayer sample for fast or slow magic angle oriented sample spinning solid-state NMR spectroscopy

被引：42

作者：

Sizun, C

Bechinger, B

机构：

[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany

[2] Inst Le Bel, F-67000 Strasbourg, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 07期

关键词：

D O I：

10.1021/ja016571o

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An alternative setup for Magic Angle Oriented Spinning Spectroscopy is proposed. Samples were prepared by orienting lipid bilayers onto polymer films, which were wrapped into a spiral so as to fit into 4 or 7 mm MAS rotors. This geometry resulted in narrow line widths and a higher upper spinning limit when compared to the conventional MAOSS setup with stacked glass plates. Whereas orientational information was extracted from low spinning spectra, fast spinning will be applicable to high-resolution multidimensional NMR pulse sequences. Copyright © 2002 American Chemical Society.

引用

收藏

页码：1146 / 1147

页数：2

相关论文

共 21 条

[1] NUCLEAR MAGNETIC RESONANCE SPECTRA FROM A CRYSTAL ROTATED AT HIGH SPEED [J].

ANDREW, ER ;

BRADBURY, A ;

EADES, RG .

NATURE, 1958, 182 (4650) :1659-1659

[2] Preparation of oriented lipid bilayer on ultrathin polymers for solid-state NMR analyses of peptide-membrane interactions [J].

Auge, S ;

Mazarguil, H ;

Tropis, M ;

Milon, A .

JOURNAL OF MAGNETIC RESONANCE, 1997, 124 (02) :455-458

[3] Towards membrane protein design: PH-sensitive topology of histidine-containing polypeptides [J].

Bechinger, B .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 263 (05) :768-775

[4]

Bechinger B, 1999, BIOPOLYMERS, V51, P174, DOI 10.1002/(SICI)1097-0282(1999)51:3<174::AID-BIP2>3.0.CO

[5]

2-7

[6]

Cross TA, 1997, METHOD ENZYMOL, V289, P672

[7] Heteronuclear 2D-correlations in a uniformly [13C, 15N] labeled membrane-protein complex at ultra-high magnetic fields [J].

Egorova-Zachernyuk, TA ;

Hollander, J ;

Fraser, N ;

Gast, P ;

Hoff, AJ ;

Cogdell, R ;

de Groot, HJM ;

Baldus, M .

JOURNAL OF BIOMOLECULAR NMR, 2001, 19 (03) :243-253

[8] Magic angle-oriented sample spinning (MAOSS): A new approach toward biomembrane studies [J].

Glaubitz, C ;

Watts, A .

JOURNAL OF MAGNETIC RESONANCE, 1998, 130 (02) :305-316

[9] Deuterium-MAS NMR spectroscopy on oriented membrane proteins:: Applications to photointermediates of bacteriorhodopsin [J].

Glaubitz, C ;

Burnett, IJ ;

Gröbner, G ;

Mason, AJ ;

Watts, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (24) :5787-5794

[10] Dipolar recoupling in MAS spectra of biological solids [J].

Griffin, RG .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (Suppl 7) :508-512