Chromosome markers in the tetraploid wheat Aegilops ventricosa analysed by in situ hybridization

被引：27

作者：

Bardsley, D

Cuadrado, A

Jack, P

Harrison, G

Castilho, A

Heslop-Harrison, JS ^{[1
]}

机构：

[1] John Innes Ctr, Dept Cell Biol, Norwich NR4 7UH, Norfolk, England

[2] Univ Alcala de Henares, Dept Biol Celular & Genet, E-28871 Alcala De Henares, Spain

[3] Plant Breeding Int, Cambridge CB2 2LQ, England

来源：

THEORETICAL AND APPLIED GENETICS | 1999年 / 99卷 / 1-2期

关键词：

karyotype evolution; genome evolution; in situ hybridization; plant breeding; alien genes; Triticum ventricosum;

D O I：

10.1007/s001220051236

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Three lines of the tetraploid wheat Aegilops ventricosa Tausch (2n = 4x = 28), which contains good resistance to eyespot, were analysed using fluorescent insitu hybridization. Probes used included rDNA, cloned repeated sequences from wheat and rye, simple-sequence repeats (SSRs) and total genomic DNA. The banding patterns produced could be used to distinguish most chromosome arms and will aid in the identification of Ac. ventricosa chromosomes or chromosome segments in breeding programmes. All lines had a single major 18S-25S rDNA site, the nucleolar organizing region (NOR) in chromosome 5N and several minor sites of 18S-25S rDNA and 5S rDNA. A 1NL.3DL, 1NS.3DS translocation was identified, and other minor differences were found between the lines.

引用

页码：300 / 304

页数：5

共 27 条

[1] Molecular cytogenetic analysis of tetraploid and hexaploid Aegilops crassa [J].