Metabolic Differences in Microbial Cell Populations Revealed by Nanophotonic Ionization

被引：56

作者：

Walker, Bennett N. ^{[1
]}

Antonakos, Cory ^{[1
]}

Retterer, Scott T. ^{[2
]}

Vertes, Akos ^{[1
]}

机构：

[1] George Washington Univ, Dept Chem, Washington, DC 20052 USA

[2] Oak Ridge Natl Lab, Oak Ridge, TN 37831 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 13期

关键词：

cellular differences; mass spectrometry; metabolites; nanostructures; single-cell analysis; SACCHAROMYCES-CEREVISIAE; MASS-SPECTROMETRY; QUANTITATIVE-ANALYSIS; FUNCTIONAL GENOMICS; YEAST; MS; RECONSTRUCTION; PHENOTYPE; CYTOMETRY; MALDI;

D O I：

10.1002/anie.201207348

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Playing (yeast) laser tag: Laser desorption ionization mass spectrometry (LDI-MS) with nanopost arrays (NAPA, see scheme) was shown to quantitatively analyze trace amounts of intracellular metabolites from single cells of Saccharomyces cerevisiae (baker's yeast). The upregulation and downregulation of metabolites because of external stress (such as oxidative stress) are also observed, even at the single-cell level. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：3650 / 3653

页数：4

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