A protocol for high-throughput extraction of DNA from rice leaves

被引：34

作者：

Xu, X

Kawasaki, S

Fujimura, T

Wang, CT ^{[1
]}

机构：

[1] S Cent Univ Natl, Key Lab Biotechnol Natl Commiss Natl, Coll Life Sci, Wuhan 430074, Peoples R China

[2] Natl Inst Agrobiol Sci, Dept Plant Physiol, Tsukuba, Ibaraki 3058602, Japan

[3] Univ Tsukuba, Lab Plant Genet Engn, Tsukuba, Ibaraki 3058572, Japan

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2005年 / 23卷 / 03期

关键词：

DNA isolation; map-based cloning; PCR amplification; rice;

D O I：

10.1007/BF02772759

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Current DNA isolation methods have limitations between speed and purity in high-throughput molecular genetic analysis such as gene mapping and marker-assisted selection programs. We have optimized a simple and rapid method for isolating high-quality genomic DNA from rice that significantly minimizes time and the use of laboratory materials. One person can process as many as 384 samples in 2 h. The isolated DNA is suitable for polymerase chain reaction-based techniques and is stable for no less than 6 mo of storage at 4 degrees C.

引用

页码：291 / 295

页数：5

共 14 条

[1] Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques [J].