Real-time PCR methods for independent quantitation of TTV and TLMV

被引：34

作者：

Moen, EM ^{[1
]}

Sleboda, J ^{[1
]}

Grinde, B ^{[1
]}

机构：

[1] Natl Inst Publ Hlth, Dept Virol, N-0403 Oslo, Norway

来源：

JOURNAL OF VIROLOGICAL METHODS | 2002年 / 104卷 / 01期

关键词：

TTV; TLMV; real-time PCR; quantitation; circovirus;

D O I：

10.1016/S0166-0934(02)00039-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

There is considerable interest in the possible clinical effects of the human circoviruses TT virus (TTV) and TTV-like mini virus (TLMV). Most people appear to have at least one of these viruses replicating actively in their bodies, thus mere correlation of the presence of virus and disease states are probably less informative than a quantitative analysis of viraemia. Real-time PCR based methods, with either SYBR Green or TaqMan probe, designed to quantitate selectively TTV and TLMV are described. The suggested TaqMan-based protocols were suitable for quantitation of viruses in the range of 10(2)-10(9) copies/ml of sample, and proved, by sequencing of PCR products, to be specific for each of the two viruses. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：59 / 67

页数：9

共 29 条

[1] Quantification of viral load: Clinical relevance for human immunodeficiency virus, hepatitis B virus and hepatitis C virus infection [J].