Polypyrrole-hydrogel composites for the construction of clinically important biosensors

被引:148
作者
Brahim, S
Narinesingh, D
Guiseppi-Elie, A [1 ]
机构
[1] Virginia Commonwealth Univ, Dept Chem Engn, Richmond, VA 23284 USA
[2] Virginia Commonwealth Univ, Ctr Bioelect Biosensors & Biochips, Richmond, VA 23284 USA
[3] Univ W Indies, Dept Chem, St Augustine, Trinidad Tobago
关键词
p(HEMA); polypyrrole; amperometric biosensor; glucose oxidase; cholesterol oxidase; galactose oxidase;
D O I
10.1016/S0956-5663(01)00262-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The present study reports on the use of p(2-hydroxyethyl methacrylate) (pHEMA) in which polypyrrole and various oxidoreductase enzymes were physically entrapped to function as a viable matrix for the construction of clinically important amperometric biosensors. Glucose oxidase. cholesterol oxidase and galactose oxidase biosensors were constructed. Electrode-supported hydrogel films were prepared by UV polymerization of the HEMA component (containing the dissolved enzyme) followed immediately by electrochemical polymerization (+ 0.7V vs. Ag/AgCl) of the pyrrole component within the interstitial spaces of the pre-formed hydrogel network. The optimized glucose oxidase biosensor displayed a wide linear glucose response range (5.0 x 10(-5) to 2.0 x 10(-2) M), a detection limit (3S(y/x)/sensitivity) of 25 muM and a response time of 35-40 s. The analytical recovery of glucose in serum samples ranged from 98 to 102% with mean coefficients of variation of 4.4% (within-day analyses) and 5.1% (day-to-day analyses). All three sensors displayed good stabilities when stored desiccated in the absence of buffer (> 9 months). (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:53 / 59
页数:7
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