Evidence for many resolvable structures within conformation types of electrosprayed ubiquitin ions

被引:137
作者
Koeniger, SL [1 ]
Merenbloom, SI [1 ]
Clemmer, DE [1 ]
机构
[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA
关键词
D O I
10.1021/jp056165h
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A new two-dimensional ion mobility spectrometry approach combined with mass spectrometry has been used to examine ubiquitin ions in the gas phase. In this approach ions are separated in an initial drift tube into conformation types (defined by their collision cross sections) and then a gate is used to introduce a narrow distribution of mobility-separated ions into a second drift tube for subsequent separation. The results show that upon selection a narrow peak shape is retained through the second drift tube. This requires that at 300 K the selected distribution does not interconvert Substantially within the broader range of structures associated with the conformation type within the similar to 10-20 ms time scale of these experiments. For the [M + 7H](7+) ion, it appears that many (similar to 5-10) narrow selections can be made across each of the compact, partially-folded, and elongated conformer types, defined previously.
引用
收藏
页码:7017 / 7021
页数:5
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