Anti-SARS-CoV immunity induced by a novel CpG oligodeoxynucleotide

被引：22

作者：

Bao, MS

Zhang, Y

Wan, M

Dai, L

Hu, XP

Wu, XL

Wang, L

Deng, P

Wang, JZ

Chen, JZ

Liu, YJ

Yu, YL ^{[1
]}

Wang, LY

机构：

[1] Jilin Univ, Dept Mol Biol, Coll Basic Med, Changchun 130021, Peoples R China

[2] Jilin Univ, Dept Immunol, Coll Basic Med, Changchun 130021, Peoples R China

[3] Natl Inst Control Pharmaceut & Biol Prod, Beijing 100050, Peoples R China

[4] Blood Ctr Jilin Province, Changchun 130033, Peoples R China

[5] MIT, Canc Res Ctr, Cambridge, MA 02139 USA

[6] MIT, Dept Biol, Cambridge, MA 02139 USA

[7] Univ Texas, MD Anderson Canc Ctr, Dept Immunol, Houston, TX 77030 USA

[8] Univ Texas, MD Anderson Canc Ctr, Ctr Canc Immunool Res, Houston, TX 77030 USA

来源：

CLINICAL IMMUNOLOGY | 2006年 / 118卷 / 2-3期

基金：

中国国家自然科学基金;

关键词：

CpG ODN; SARS-CoV; anit-virus; PBMCs; pDCs; B cells; NK cells; IFN-alpha; IFN-gamma;

D O I：

10.1016/j.clim.2005.09.014

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

To develop CpG oligodeoxynucleotides (CpG ODNs) based therapy for prevention and treatment of severe acute respiratory syndrome (SARS), we selected a novel CpG ODN (BW001), which displays B-type CpG ODN structure feature at the 5' and A-type CpG ODN structure feature at the 3' and tested for its anti-SARS-CoV activity. We found that the supernatants of human PBMCs stimulated by BW001 significantly protected Vero cells from SARS-CoV infection. BW001 could stimulate human PBMCs and pDCs to secrete high level of IFN-alpha and promote human PBMCs and B cells to proliferate. Furthermore, we demonstrated that BW001 could activate CD19(+) B cells and CD56(+) NK cells in human PBMCs. In addition, BW001 could enhance NK cytotoxicity and IFN-gamma secretion in human PBMCs. Together, BW001 represents a novel type of CpG ODN and may have potential for the development of treatment and prevention for SARS as well as other viral associated diseases. (c) 2005 Published by Elsevier Inc.

引用

页码：180 / 187

页数：8

共 30 条

[1]

Inhibition of human herpes simplex virus type 2 by interferon γ and tumor necrosis factor α is mediated by indoleamine 2,3-dioxygenase [J].