Up-regulation of PDCD4 in senescent human diploid fibroblasts

被引：48

作者：

Kang, MJ

Ahn, HS

Lee, JY

Matsuhashi, S

Park, WY

机构：

[1] Seoul Natl Univ, Coll Med, Dept Biochem & Mol Biol, Chongnogu, Seoul 110799, South Korea

[2] Seoul Natl Univ, Coll Med, Ilchun Mol Med Inst, Chongnogu, Seoul 110799, South Korea

[3] Saga Med Sch, Dept Internal Med, Saga 849, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 293卷 / 01期

关键词：

PDCD4; senescence; EIF4G; ribosomal proteins;

D O I：

10.1016/S0006-291X(02)00264-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Programmed cell death 4 (PDCD4) has a common MI domain sharing with death associated protein 5 (DAP5) and a component of eukaryotic translation initiation factor (eIF4G) complex and it might also work as a tumor suppressor. We could find that the message and product of Pdcd4 gene were up-regulated in senescent human diploid fibroblasts. In yeast two hybrid analysis, the C-terminal region of PDCD4 interacted with ribosomal protein S13 (RPS13). ribosomal protein L5 (RPL5), and TI-227H. In in vitro binding assay, RPS13. a component of 40S ribosome was stably bound to PDCD4. We also found that PDCD4 was localized to polysome fractions. We could pull out eIF4G with GST-PDCD4. but eIF4E did not interact with PDCD4. From these results, we could assume that PDCD4 might regulate the eIF4G-dependent translation through direct interactions with eIF4G and RPS13 in senescent fibroblasts. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：617 / 621

页数：5

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