On-line monitoring and identification of bioaerosols

被引：69

作者：

Rösch, P

Harz, M

Peschke, KD

Ronneberger, O

Burkhardt, H

Schule, A

Schmauz, G

Lankers, M

Hofer, S

Thiele, H

Motzkus, HW

Popp, J

机构：

[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[2] Univ Freiburg, Inst Informat, Lehrstuhl Mustererkennung & Bildverarbeitung, D-79110 Freiburg, Germany

[3] Fraunhofer Inst Produktionstechn & Automatisierun, D-70569 Stuttgart, Germany

[4] Rap ID Particle Syst GmbH, D-12459 Berlin, Germany

[5] Kayser Threde GmbH, D-81379 Munich, Germany

[6] Schering AG, D-13353 Berlin, Germany

[7] Inst Phys Hochtechnol, D-07745 Jena, Germany

来源：

ANALYTICAL CHEMISTRY | 2006年 / 78卷 / 07期

关键词：

D O I：

10.1021/ac0514974

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fast analysis of bioaerosols in clean room environments is necessary in order to prevent contamination of pharmaceutical products, minimize machine downtimes, or both. The detection and identification of microbes will be carried out in several steps: After impaction of the aerosol on a surface, the particles are presorted with glancing fight illumination and fluorescence imaging in order to distinguish between abiotic and biotic particles. Since only the biotic particles are of interest, the analysis time can be minimized due to reduction of the data set. The biotic particles are then analyzed further with Raman spectroscopy and identified with a support vector machine.

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收藏

页码：2163 / 2170

页数：8

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