Expression of voltage-dependent chloride channels in the rat cochlea

被引：12

作者：

Oshima, T

Ikeda, K

Furukawa, M

Takasaka, T

机构：

[1] Department of Otolaryngology, Tohoku University, School of Medicine, Aoba-ku, Sendai 980-77

来源：

HEARING RESEARCH | 1997年 / 103卷 / 1-2期

关键词：

chloride channel; voltage-dependent chloride channel; RT-PCR; cochlea; (rat);

D O I：

10.1016/S0378-5955(96)00164-5

中图分类号：

R36 [病理学]; R76 [耳鼻咽喉科学];

学科分类号：

100104 [病理学与病理生理学]; 100213 [耳鼻咽喉科学];

摘要：

Voltage-dependent chloride (ClC) channels have not yet been identified in the cochlea. In this study, an approach utilizing the reverse transcription-polymerase chain reaction (RT-PCR) was devised to clone the cDNA of CIC channels. PCR was performed using degenerate primers corresponding to two highly conserved regions of the CIC channels. By Southern hybridization and sequencing studies, the sequences corresponding to ClC-2 and ClC-3 were found in the cochlear lateral wall, while ClC-1 was not detected. These results suggest that ClC-2 and ClC-3 might be involved in Cl- transport in the cochlear lateral wall.

引用

页码：63 / 68

页数：6

共 31 条

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[3]

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