A functional bipartite nuclear localisation signal in the cytokine interleukin-5

被引:39
作者
Jans, DA [1 ]
Briggs, LJ [1 ]
Gustin, SE [1 ]
Jans, P [1 ]
Ford, S [1 ]
Young, IG [1 ]
机构
[1] AUSTRALIAN NATL UNIV, JOHN CURTIN SCH MED RES, DIV BIOCHEM & MOL BIOL, MED MOL BIOL GRP, CANBERRA, ACT 2601, AUSTRALIA
来源
FEBS LETTERS | 1997年 / 406卷 / 03期
关键词
interleukin-5; nuclear protein import; confocal laser scanning microscopy; microinjection; cytokine receptor;
D O I
10.1016/S0014-5793(97)00293-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Interleukin (IL)-5 is central in regulating eosinophilia in allergic disease and parasitic infections, We have identified a bipartite nuclear localisation signal (NLS) within amino acids 95-111 of human IL-5 (hIL-5), also present in mouse IL-5 (mIL-5), hIL-5 and mIL-5 were labelled fluorescently, and nuclear uptake subsequent to membrane binding and internalisation by intact receptor expressing cells visualised and quantified using confocal laser scanning microscopy, hIL-5 and mIL-5 were shown to be transported to the nucleus in in vivo and in vitro nuclear protein import assays, The hIL-5 NLS was able to target a heterologous protein to the nucleus both in vivo and in vitro, Mutations within the proximal arm of the NLS abrogated nuclear targeting activity, confirming its bipartite nature, The results imply a nuclear signalling role for IL-5 additional to pathways linked to the membrane receptor system. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码:315 / 320
页数:6
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