Membrane proteomics of phagosomes suggests a connection to autophagy

被引:91
作者
Shui, Wenqing [2 ]
Sheu, Leslie [3 ]
Liu, Jun [6 ]
Smart, Brian [2 ]
Petzold, Christopher J. [1 ]
Hsieh, Tsung-yen [3 ]
Pitcher, Austin [2 ]
Keasling, Jay D. [1 ,4 ,7 ]
Bertozzi, Carolyn R. [2 ,3 ,5 ]
机构
[1] Univ Calif Berkeley, Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[4] Univ Calif Berkeley, Dept Chem Engn & Bioengn, Berkeley, CA 94720 USA
[5] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[6] Bayer HealthCare LLC, Biol Prod Div, Berkeley, CA 94701 USA
[7] Joint BioEnergy Inst, Emeryville, CA 94720 USA
基金
美国国家卫生研究院;
关键词
LC3; phagocytosis;
D O I
10.1073/pnas.0809218105
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Phagocytosis is the central process by which macrophage cells internalize and eliminate infectious microbes as well as apoptotic cells. During maturation, phagosomes containing engulfed particles fuse with various endosomal compartments through the action of regulatory molecules on the phagosomal membrane. In this study, we performed a proteomic analysis of the membrane fraction from latex bead-containing (LBC) phagosomes isolated from macrophages. The profile, which comprised 546 proteins, suggests diverse functions of the phagosome and potential connections to secretory processes, toll-like receptor signaling, and autophagy. Many identified proteins were not previously known to reside in the phagosome. We characterized several proteins in LBC phagosomes that change in abundance on induction of autophagy, a process that has been previously implicated in the host defense against microbial pathogens. These observations suggest crosstalk between autophagy and phagocytosis that may be relevant to the innate immune response of macrophages.
引用
收藏
页码:16952 / 16957
页数:6
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