Evaluation of the use of RT-PCR for the early diagnosis of dengue fever

被引：16

作者：

Grobusch, MP

Niedrig, M

Göbels, K

Klipstein-Grobusch, K

Teichmann, D

机构：

[1] Humboldt Univ, Med Clin, Dept Infect Dis, Charite, D-1086 Berlin, Germany

[2] German Inst Human Nutr, DIfE, Nuthetal, Germany

[3] Robert Koch Inst, D-1000 Berlin, Germany

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2006年 / 12卷 / 04期

关键词：

dengue fever; diagnosis; RT-PCR; sampling time; sensitivity;

D O I：

10.1111/j.1469-0691.2006.01353.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

RT-PCR was used to diagnose dengue virus infections confirmed serologically in 26 returning travellers. RT-PCR was positive for three (75%) of four samples taken on or before day 3 of the illness, for 15 (78.9%) of 19 samples taken between days 4 and 7, and for none of three samples tested on or after day 8 (p 0.0337). When applied early, RT-PCR seems to be a useful tool for the diagnosis of dengue fever.

引用

收藏

页码：395 / 397

页数：3

相关论文

共 13 条

[1] The use of reverse transcription-polymerase chain reaction (RT-PCR) for the rapid detection and identification of dengue virus in an endemic region:: a validation study [J].

De Paula, SO ;

Neto, RJP ;

Corrêa, JACT ;

Assumpçao, SR ;

Costa, MLS ;

Lima, DM ;

Fonseca, BAL .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2002, 96 (03) :266-269

[2]

DOMINGO C, 2005, WHO DENGUE B, V28, P87

[3] Epidemiology and clinical features of imported dengue fever in Europe:: Sentinel surveillance data from TropNetEurop [J].

Jelinek, T ;

Müberger, NM ;

Harms, G ;

Corachán, M ;

Grobusch, MP ;

Knobloch, J ;

Bronner, U ;

Laferl, H ;

Kapaun, A ;

Bisoffi, Z ;

Clerinx, J ;

Puente, S ;

Fry, G ;

Schulze, M ;

Hellgren, U ;

Gjorup, I ;

Chalupa, P ;

Hatz, C ;

Matteelli, A ;

Schmid, M ;

Nielsen, LN ;

da Cunha, S ;

Atouguia, J ;

Myrvang, B ;

Fleischer, K .

CLINICAL INFECTIOUS DISEASES, 2002, 35 (09) :1047-1052

[4] External quality control assessment in PCR diagnostics of dengue virus infections [J].

Lemmer, A ;

Mantke, OD ;

Bae, HG ;

Groen, J ;

Drosten, C ;

Niedrig, M .

JOURNAL OF CLINICAL VIROLOGY, 2004, 30 (04) :291-296

[5] Dengue: an arthropod-borne disease of global importance [J].

Mairuhu, ATA ;

Wagenaar, J ;

Brandjes, DPM ;

van Gorp, ECM .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2004, 23 (06) :425-433

[6] Quality control assessment for the serological diagnosis of dengue virus infections [J].

Mantke, OD ;

Lemmer, K ;

Biel, SS ;

Groen, J ;

Schmitz, H ;

Durand, JP ;

Zeller, H ;

Niedrig, M .

JOURNAL OF CLINICAL VIROLOGY, 2004, 29 (02) :105-112

[7] Rapid diagnosis of Dengue viremia by reverse transcriptase-polymerase chain reaction using 3'-noncoding region universal primers [J].

Sudiro, TM ;

Ishiko, H ;

Green, S ;

Vaughn, DW ;

Nisalak, A ;

Kalayanarooj, S ;

Rothman, AL ;

Raengsakulrach, B ;

Janus, J ;

Kurane, I ;

Ennis, FA .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1997, 56 (04) :424-429

[8] Dengue virus infection in travellers returning to Berlin, Germany:: clinical, laboratory, and diagnostic aspects [J].

Teichmann, D ;

Göbels, K ;

Niedrig, M ;

Grobusch, MP .

ACTA TROPICA, 2004, 90 (01) :87-95

[9] Virus isolation for diagnosing dengue virus infections in returning travelers [J].

Teichmann, D ;

Göbels, K ;

Niedrig, M ;

Sim-Brandenburg, JW ;

Làge-Stehr, J ;

Grobusch, MP .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2003, 22 (11) :697-700

[10]

ter Meulen J, 2000, TROP MED INT HEALTH, V5, P325