Plasma polymerized epoxide functional surfaces for DNA probe immobilization

被引:21
作者
Chu, Li-Qiang [1 ]
Knoll, Wolfgang [1 ]
Foerch, Renate [1 ]
机构
[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany
关键词
DNA sensor; plasma polymerization; epoxide functional surface; surface plasmon enhanced fluorescence spectroscopy (SPFS);
D O I
10.1016/j.bios.2008.03.026
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The development of functional surfaces for the immobilization of DN probe is crucial for a successful design of a DNA sensor. In this report, epoxide functional thin films were achieved simply by pulsed plasma polymerization (PP) of glycidyl methacrylate (GMA) at low duty cycle. The presence of epoxide groups in the resulting ppGMA films was confirmed by Fourier transform infrared spectroscopy. The ppGMA coatings were found to be resistant to the non-specific adsorption of DNA strands, while the epoxide groups obtained could react with amine-modified DNA probes in a mild basic environment without any activation steps. A DNA sensor was made, and was successfully employed to distinguish different DNA sequences with one base pair mismatch as seen by Surface plasmon enhanced fluorescence spectroscopy (SPFS). The regeneration of the present DNA sensor was also discussed. This result suggests that surface modification with ppGMA films is very promising for the fabrication of various DNA sensors. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:118 / 122
页数:5
相关论文
共 27 条
  • [1] Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111)
    Böcking, T
    Killan, KA
    Gaus, K
    Gooding, JJ
    [J]. LANGMUIR, 2006, 22 (08) : 3494 - 3496
  • [2] N-(3-triethoxysilylpropyl)-6-(N-maleimido)-hexanamide:: An efficient heterobifunctional reagent for the construction of oligonucleotide microarrays
    Choithani, Jyoti
    Kumar, P.
    Gupta, K. C.
    [J]. ANALYTICAL BIOCHEMISTRY, 2006, 357 (02) : 240 - 248
  • [3] Pulsed plasma polymerized di(ethylene glycol) monovinyl ether coatings for nonfouling surfaces
    Chu, Li-Qiang
    Knoll, Wolfgang
    Foerch, Renate
    [J]. CHEMISTRY OF MATERIALS, 2006, 18 (20) : 4840 - 4844
  • [4] Biologically multifunctional surfaces using plasma polymerization methods
    Chu, Liqiang
    Knoll, Wolfgang
    Foerch, Renate
    [J]. PLASMA PROCESSES AND POLYMERS, 2006, 3 (6-7) : 498 - 505
  • [5] Plasma-surface modification of biomaterials
    Chu, PK
    Chen, JY
    Wang, LP
    Huang, N
    [J]. MATERIALS SCIENCE & ENGINEERING R-REPORTS, 2002, 36 (5-6) : 143 - 206
  • [6] MultiFunctional molecular scratchcards
    Harris, L. G.
    Schofield, W. C. E.
    Badyal, J. P. S.
    [J]. CHEMISTRY OF MATERIALS, 2007, 19 (07) : 1546 - 1551
  • [7] Immobilization of DNA on microarrays
    Heise, C
    Bier, FF
    [J]. IMMOBILISATION OF DNA ON CHIPS II, 2005, 261 : 1 - 25
  • [8] Investigating the kinetics of DNA-DNA and PNA-DNA interactions using surface plasmon resonance-enhanced fluorescence spectroscopy
    Kambhampati, D
    Nielsen, PE
    Knoll, W
    [J]. BIOSENSORS & BIOELECTRONICS, 2001, 16 (9-12) : 1109 - 1118
  • [9] DIRECT-DETECTION OF NUCLEIC-ACID HYBRIDIZATION ON THE SURFACE OF A CHARGE-COUPLED-DEVICE
    LAMTURE, JB
    BEATTIE, KL
    BURKE, BE
    EGGERS, MD
    EHRLICH, DJ
    FOWLER, R
    HOLLIS, MA
    KOSICKI, BB
    REICH, RK
    SMITH, SR
    VARMA, RS
    HOGAN, ME
    [J]. NUCLEIC ACIDS RESEARCH, 1994, 22 (11) : 2121 - 2125
  • [10] Complement hybridization from solution to surface-attached probe-oligonucleotides observed by surface-plasmon-field-eahanced fluorescence spectroscopy
    Liebermann, T
    Knoll, W
    Sluka, P
    Herrmann, R
    [J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2000, 169 (1-3) : 337 - 350