Single-step DNA immobilization on antifouling self-assembled monolayers covalently bound to silicon (111)

被引：66

作者：

Böcking, T

Killan, KA

Gaus, K

Gooding, JJ ^{[1
]}

机构：

[1] Univ New S Wales, Sch Chem, Sydney, NSW 2052, Australia

[2] Univ New S Wales, Sch Phys, Sydney, NSW 2052, Australia

[3] Univ New S Wales, Sch Med Sci, Ctr Vasc Res, Sydney, NSW 2052, Australia

来源：

LANGMUIR | 2006年 / 22卷 / 08期

关键词：

D O I：

10.1021/la060331a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Hydrosilylation of alkenes with epoxide-terminated tri(ethylene oxide) moieties on Si-H surfaces yields homogeneous monolayers for the efficient coupling of biomolecules. The wetting properties of the epoxide-functionalized surface allow for the spotting of solutions of biomolecules, making the surface amenable to microarraying. Immobilization of thiolated DNA was achieved in a single step to fabricate biorecognition interfaces showing the hybridization of complementary DNA at low concentrations and negligible binding of noncomplementary DNA.

引用

收藏

页码：3494 / 3496

页数：3

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