In vivo veritas: electron cryotomography of cells

被引:22
作者
Plitzko, Juergen M. [1 ]
Frangakis, Achilleas S. [1 ]
Nickell, Stephan [1 ]
Foerster, Friedrich [1 ]
Gross, Ariane [1 ]
Baumeister, Wolfgang [1 ]
机构
[1] Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany
关键词
D O I
10.1016/S0167-7799(02)02017-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Automated electron cryotomography enables the study of organelles or whole cells embedded in vitrified ice in a close-to-life state and with a resolution of a few nanometers. This technology will provide new vistas of the supramolecular architecture inside cells that orchestrates higher cellular functions.
引用
收藏
页码:S40 / S44
页数:5
相关论文
共 29 条
[21]   Application of electron tomography to mitochondrial research [J].
Mannella, CA .
METHODS IN CELL BIOLOGY, VOL 65: MITOCHONDRIA, 2001, 65 :245-+
[22]   Dual-axis tomography: An approach with alignment methods that preserve resolution [J].
Mastronarde, DN .
JOURNAL OF STRUCTURAL BIOLOGY, 1997, 120 (03) :343-352
[23]   THE RELEVANCE OF DOSE-FRACTIONATION IN TOMOGRAPHY OF RADIATION-SENSITIVE SPECIMENS [J].
MCEWEN, BF ;
DOWNING, KH ;
GLAESER, RM .
ULTRAMICROSCOPY, 1995, 60 (03) :357-373
[24]   Electron microscopy of cells: A new beginning for a new century [J].
McIntosh, JR .
JOURNAL OF CELL BIOLOGY, 2001, 153 (06) :F25-F32
[25]   Cryo-electron tomography of Neurospora mitochondria [J].
Nicastro, D ;
Frangakis, AS ;
Typke, D ;
Baumeister, W .
JOURNAL OF STRUCTURAL BIOLOGY, 2000, 129 (01) :48-56
[26]   DOUBLE-TILT ELECTRON TOMOGRAPHY [J].
PENCZEK, P ;
MARKO, M ;
BUTTLE, K ;
FRANK, J .
ULTRAMICROSCOPY, 1995, 60 (03) :393-410
[27]   ON THE DETERMINATION OF FUNCTIONS FROM THEIR INTEGRAL VALUES ALONG CERTAIN MANIFOLDS [J].
RADON, J .
IEEE TRANSACTIONS ON MEDICAL IMAGING, 1986, 5 (04) :170-176
[28]   3-DIMENSIONAL RECONSTRUCTION OF IMPERFECT TWO-DIMENSIONAL CRYSTALS [J].
SAXTON, WO ;
BAUMEISTER, W ;
HAHN, M .
ULTRAMICROSCOPY, 1984, 13 (1-2) :57-70
[29]   The evolving complexity of cytoplasmic structure [J].
Schliwa, M .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2002, 3 (04) :291-296