A strategy for examining complex mixtures of deoxyoligonucleotides using ion-pair-reverse-phase high-performance liquid chromatography, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, and informatics

被引：7

作者：

Vallone, PM

Devaney, JM

Marino, MA

Butler, JM

机构：

[1] Natl Inst Stand & Technol, Div Biotechnol, Gaithersburg, MD 20899 USA

[2] Transgenom Inc, Gaithersburg, MD 20878 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 304卷 / 02期

关键词：

D O I：

10.1006/abio.2002.5641

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and ionpair-reverse-phase high-performance liquid chromatography (IP-RP HPLC) techniques were combined to determine the sequence identity of short single-stranded deoxyoligonucleotides. This methodology is demonstrated using a commercially available multiplex set of eight primer pairs. The primer pairs were separated and collected by IP-RP HPLC. Partial sequence information for IP-RP HPLC fractions was obtained from analyzing exonuclease digestion products by MALDI-TOF MS. IP-RP HPLC, MALDI-TOF MS, exonuclease digests, and a simple computational algorithm provide an integrated strategy for determining the sequence of short nucleic acid oligomers. (C) 2002 Elsevier Science (USA).

引用

页码：257 / 265

页数：9

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