Nardosinone Protects H9c2 Cardiac Cells from Angiotensin II-induced Hypertrophy

被引:27
作者
Du, Meng [1 ,2 ]
Huang, Kun [1 ,2 ]
Gao, Lu [2 ]
Yang, Liu [2 ]
Wang, Wen-shuo [3 ]
Wang, Bo [3 ]
Huang, Kai [1 ,2 ]
Huang, Dan [1 ,2 ]
机构
[1] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Clin Ctr Human Genom Res, Wuhan 430022, Peoples R China
[2] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Cardiovasc Dis, Wuhan 430022, Peoples R China
[3] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Cardiovasc Surg, Wuhan 430022, Peoples R China
基金
中国国家自然科学基金;
关键词
nardosinone; cardiac hypertrophy; H9c2; cells; PI3K/Akt; MEK/ERK; NERVE GROWTH-FACTOR; NEURITE OUTGROWTH; PC12D CELLS; NARDOSTACHYS-CHINENSIS; SIGNALING PATHWAYS; HEART-FAILURE; ACTIVATION; RECEPTOR; ENHANCER; MICE;
D O I
10.1007/s11596-013-1205-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Pathological cardiac hypertrophy induced by angiotensin. (Ang II) can subsequently give rise to heart failure, a leading cause of mortality. Nardosinone is a pharmacologically active compound extracted from the roots of Nardostachys chinensis, a well-known traditional Chinese medicine. In order to investigate the effects of nardosinone on Ang II-induced cardiac cell hypertrophy and the related mechanisms, the myoblast cell line H9c2, derived from embryonic rat heart, was treated with nardosinone (25, 50, 100, and 200 mu mol/L) or Ang. (1 mu mol/L). Then cell surface area and mRNA expression of classical markers of hypertrophy were detected. The related protein levels in PI3K/Akt/mTOR and MEK/ERK signaling pathways were examined by Western blotting. It was found that pretreatment with nardosinone could significantly inhibit the enlargement of cell surface area induced by Ang.. The mRNA expression of ANP, BNP and beta-MHC was obviously elevated in Ang II-treated H9c2 cells, which could be effectively blocked by nardosinone at the concentration of 100 mu mol/L. Further study revealed that the protective effects of nardosinone might be mediated by repressing the phosphorylation of related proteins in PI3K/Akt and MEK/ERK signaling pathways. It was suggested that the inhibitory effect of nardosinone on Ang II-induced hypertrophy in H9c2 cells might be mediated by targeting PI3K/Akt and MEK/ERK signaling pathways.
引用
收藏
页码:822 / 826
页数:5
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