Quantification in comprehensive two-dimensional liquid chromatography

被引：42

作者：

Mondello, Luigi ^{[1
,2
]}

Herrero, Miguel ^{[1
]}

Kumm, Tiina ^{[1
]}

Dugo, Paola ^{[3
]}

Cortes, Hernan ^{[4
]}

Dugo, Giovanni ^{[1
]}

机构：

[1] Univ Messina, Facolta Farm, Dipartimento Farmacochim, I-98168 Messina, Italy

[2] Campus Biomed, I-00155 Rome, Italy

[3] Univ Messina, Facolta Sci, Dipartimento Sci Alimenti & Ambiente, I-98166 Messina, Italy

[4] Dow Chem Co USA, Analyt Sci, Midland, MI 48667 USA

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 14期

关键词：

D O I：

10.1021/ac800484y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Although the use of comprehensive two-dimensional liquid chromatography (LC x LC) as a powerful separation technique is continuously increasing, its employment in quantification experiments is rather limited. The present research is focused on the quantification of a series of standards, as well as real-world sample compounds, by using dedicated laboratory-constructed LC x LC software, developed through a novel approach. Moreover, the difficulties encountered during software operation, in various elution conditions, are described and discussed. The results attained were compared with those observed in conventional LC, and no statistically significant differences were observed in the determination of aurapten in grapefruit oil. However, a loss in sensitivity was observed when using LCxLC (limit of detection = 0.10 ppm) compared to conventional LC (limit of detection = 0.05 ppm) as a consequence of the sample dilution in comprehensive two-dimensional liquid chromatography.

引用

收藏

页码：5418 / 5424

页数：7

相关论文

共 25 条

[1] Quantification in comprehensive two-dimensional gas chromatography and a model of quantification based on selected summed modulated peaks [J].

Amador-Munoz, Omar ;

Marriott, Philip J. .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1184 (1-2) :323-340

[2] AUTOMATED INSTRUMENTATION FOR COMPREHENSIVE 2-DIMENSIONAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PROTEINS [J].

BUSHEY, MM ;

JORGENSON, JW .

ANALYTICAL CHEMISTRY, 1990, 62 (02) :161-167

[3] Temperature effects on separation on zirconia columns: Applications to one- and two-dimensional LC separations of phenolic antioxidants [J].

Cacciola, Francesco ;

Jandera, Pavel ;

Mondello, Luigi .

JOURNAL OF SEPARATION SCIENCE, 2007, 30 (04) :462-474

[4] DEVELOPMENTS IN MULTIDIMENSIONAL SEPARATION SYSTEMS [J].

CORTES, HJ .

JOURNAL OF CHROMATOGRAPHY, 1992, 626 (01) :3-23

[5] Comprehensive normal-phase x reversed-phase liquid chromatography coupled to photodiode array and mass spectrometry detection for the analysis of free carotenoids and carotenoid esters from mandarin [J].

Dugo, P. ;

Herrero, M. ;

Kumm, T. ;

Giuffrida, D. ;

Dugo, G. ;

Mondello, L. .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1189 (1-2) :196-206

[6] Comprehensive two-dimensional normal-phase (adsorption) - Reversed-phase liquid chromatography [J].

Dugo, P ;

Favoino, O ;

Luppino, R ;

Dugo, G ;

Mondello, L .

ANALYTICAL CHEMISTRY, 2004, 76 (09) :2525-2530

[7] Application of comprehensive two-dimensional liquid chromatography to elucidate the native carotenoid composition in red orange essential oil [J].

Dugo, Paola ;

Herrero, Miguel ;

Giuffrida, Daniele ;

Kumm, Tiina ;

Dugo, Giovanni ;

Mondello, Luigi .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (10) :3478-3485

[8] Comprehensive multidimensional liquid chromatography: Theory and applications [J].

Dugo, Paola ;

Cacciola, Francesco ;

Kumm, Tiina ;

Dugo, Giovanni ;

Mondello, Luigi .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1184 (1-2) :353-368

[9] Elucidation of carotenoid patterns in citrus products by means of comprehensive normal-phase x reversed-phase liquid chromatography [J].

Dugo, Paola ;

Skerikova, Veronika ;

Kumm, Tiina ;

Trozzi, Alessandra ;

Jandera, Pavel ;

Mondello, Luigi .

ANALYTICAL CHEMISTRY, 2006, 78 (22) :7743-7750

[10] Separation of triacylglycerols in a complex lipidic matrix by using comprehensive two-dimensional liquid chromatography coupled with atmospheric pressure chemical ionization mass spectrometric detection [J].

Dugo, Paola ;

Kumm, Tiina ;

Chiofalo, Biagina ;

Cotroneo, Antonella ;

Mondello, Luigi .

JOURNAL OF SEPARATION SCIENCE, 2006, 29 (08) :1146-1154