Affinity-Based Labeling of Cytohesins with a Bifunctional SecinH3 Photoaffinity Probe

被引：22

作者：

Bi, Xihe ^{[1
]}

Schmitz, Anton ^{[1
]}

Hayallah, Alaa M. ^{[1
]}

Song, Jin-Na ^{[1
]}

Famulok, Michael ^{[1
]}

机构：

[1] Univ Bonn, LIMES Inst Program Unit Chem Biol & Med Chem, D-53121 Bonn, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 49期

关键词：

benzophenone; cytohesins; inhibitors; photoaffinity labeling; proteins;

D O I：

10.1002/anie.200803962

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) The specific binding of the cytohesin inhibitor SecinH3 to Sec7 domains of cytohesins was shown by the use of a bifunctional photoaffinity probe (see structure) on guanine nucleotide exchange factors (GEFs) and GTPases. This probe should be useful for the proteome-wide profiling of cytohesin complexes and the identification of the binding site. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：9565 / 9568

页数：4

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