Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats

被引：35

作者：

Zhang, WR

Kitagawa, H

Hayashi, T

Sasaki, C

Sakai, K

Warita, H

Shiro, Y

Suenaga, H

Ohmae, H

Tsuji, S

Itoh, T

Nishimura, O

Nagasaki, H

Abe, K

机构：

[1] Okayama Univ, Sch Med, Dept Neurol, Okayama 700, Japan

[2] Takeda Chem Ind Ltd, Pharmaceut Discovery Res Div, Osaka 532, Japan

来源：

BRAIN RESEARCH | 1999年 / 842卷 / 01期

关键词：

neurotrophin-3; ischemia; MCAO; TUNEL; caspase-3; HSP72;

D O I：

10.1016/S0006-8993(99)01818-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain injury, NT-3 was topically applied to brain surface just after 90 min of middle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced the infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohistochemical study for caspase-3 and heat shock protein 72 (HSP72) showed that NT-3 treatment decreased the number of cells with DNA fragmentation and caspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuronal cells from ischemic injury, and it is possibly associated with inhibition of DNA fragmentation. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：211 / 214

页数：4

共 17 条

[11] Differential regulation of ciliary neurotrophic factor (CNTF) and CNTF receptor α (CNTFR α) expression following focal cerebral ischemia [J].