Micellar electrokinetic capillary chromatography as an alternative method for the determination of estrogens

被引：12

作者：

Berzas, JJ ^{[1
]}

Castañeda, G ^{[1
]}

Pinilla, MJ ^{[1
]}

机构：

[1] Univ Castilla La Mancha, Dept Analyt Chem & Food Technol, E-13071 Ciudad Real, Spain

来源：

ANALYTICAL LETTERS | 1999年 / 32卷 / 12期

关键词：

ethinylestradiol; gestodene; (-)norgestrel; beta-estradiol valerate; micellar electrokinetic chromatography (MEKC); estrogens;

D O I：

10.1080/00032719908542981

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A micellar electrokinetic chromatographic method which permits the separation of very similar compounds such as ethinylestradiol (ETE), gestodene (GTD), (-)norgestrel (LEV) and β-estradiol valerate (EST) from synthetic mixtures is described. The study was carried out at 25°C and 20 kV, using a 15 mM borate buffer (pH 9.2), 15 mM sodium dodecylsulfate (SDS) and 26 % acetonitrile-water as background electrolyte. The analysis takes about 12 minutes.

引用

页码：2453 / 2469

页数：17

共 21 条

[1] APPROACHES TO OPTIMIZATION OF PRECISION IN CAPILLARY ELECTROPHORESIS [J].

ALTRIA, KD ;

FABRE, H .

CHROMATOGRAPHIA, 1995, 40 (5-6) :313-320

[2] VALIDATION OF A CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF A QUINOLONE ANTIBIOTIC AND ITS RELATED IMPURITIES [J].

ALTRIA, KD ;

CHANTER, YL .

JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (02) :459-463

[3] ESSENTIAL PEAK AREA NORMALIZATION FOR QUANTITATIVE IMPURITY CONTENT DETERMINATION BY CAPILLARY ELECTROPHORESIS [J].

ALTRIA, KD .

CHROMATOGRAPHIA, 1993, 35 (3-4) :177-182

[4] QUANTITATIVE ASPECTS OF THE APPLICATION OF CAPILLARY ELECTROPHORESIS TO THE ANALYSIS OF PHARMACEUTICALS AND DRUG-RELATED IMPURITIES [J].

ALTRIA, KD .

JOURNAL OF CHROMATOGRAPHY, 1993, 646 (02) :245-257

[5]

Baker D. R., 1995, CAPILLARY ELECTROPHO

[6] Determination of ethinylestradiol and gestodene in oral contraceptives by micellar electrokinetic chromatography [J].

Berzas, JJ ;

Rodríguez, J ;

Castañeda, G ;

Pinilla, MJ .

CHROMATOGRAPHIA, 1999, 49 (1-2) :65-70

[7]

BERZAS JJ, IN PRESS ANAL SCI

[8]

Berzas Nevado J. J., 1997, Analytical Letters, V30, P2221

[9]

CASTANEDAPENALV.G, 1996, CHROMATOGRAPHIA, V42, P159

[10] PROBLEMS OF QUANTITATIVE INJECTION IN CAPILLARY ZONE ELECTROPHORESIS [J].

DOSE, EV ;

GUIOCHON, G .

ANALYTICAL CHEMISTRY, 1992, 64 (02) :123-128

← 1 2 3 →