PhaQ, a new class of poly-β-hydroxybutyrate (PHB)-responsive repressor, regulates phaQ and phaP (phasin) expression in Bacillus megaterium through interaction with PHB

被引：28

作者：

Lee, TR ^{[1
]}

Lin, JS ^{[1
]}

Wang, SS ^{[1
]}

Shaw, GC ^{[1
]}

机构：

[1] Natl Yang Ming Univ, Sch Life Sci, Inst Biochem, Taipei 112, Taiwan

来源：

JOURNAL OF BACTERIOLOGY | 2004年 / 186卷 / 10期

关键词：

D O I：

10.1128/JB.186.10.3015-3021.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus megaterium can produce poly-beta-hydroxybutyrate (PHB) as carbon and energy storage materials. We now report that the phaQ gene, which is located upstream of the phasin-encoding phaP gene, codes for a new class of transcriptional regulator that negatively controls expression of tooth phaQ and phaP. A PhaQ binding site that plays a role in this control has been identified by gel mobility shift assays and DNase I footprinting analysis. We have also provided evidence that PhaQ could sense the presence of PHB in vivo and that artificial PHB granules could inhibit the formation of PhaQ-DNA complex in vitro by binding to PhaQ directly. These suggest that PhaQ is a PHB-responsive repressor.

引用

页码：3015 / 3021

页数：7

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