RuBiGABA-2: a hydrophilic caged GABA with long wavelength sensitivity

被引：34

作者：

Filevich, Oscar ^{[1
]}

Etchenique, Roberto ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Inorgan Analit & Quim Fis, INQUIMAE, Buenos Aires, DF, Argentina

来源：

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2013年 / 12卷 / 09期

关键词：

LIGHT; PHOTORELEASE; 2-PHOTON; NEUROTRANSMITTER; PHOTOCHEMISTRY; GLUTAMATE; RELEASE;

D O I：

10.1039/c3pp25248e

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

We have devised a new caged GABA based on ruthenium bipyridyl coordination chemistry. This photo-trigger delivers GABA upon irradiation with wavelengths up to 532 nm undergoing heterolytic photo-cleavage, in a clean and very fast (a few nanoseconds) photoreaction. With an absorptivity coefficient epsilon(MAX) = 5300 M-1 cm(-1) at 447 nm and a quantum efficiency phi similar to 0.09, RuBiGABA-2 is among the most active caged-GABAs, especially at long wavelengths. This highly hydrophilic caged GABA can be synthesized in a simple one-pot reaction. The synthesis, chemical characterization and photochemical properties are presented. Finally, the usefulness of this caged compound is demonstrated by photodelivering free GABA on leech motoneurons.

引用

页码：1565 / 1570

页数：6

共 22 条

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