Caged compounds: photorelease technology for control of cellular chemistry and physiology

被引：731

作者：

Ellis-Davies, Graham C. R. ^{[1
]}

机构：

[1] Drexel Univ, Coll Med, Dept Physiol & Pharmacol, Philadelphia, PA 19102 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 08期

关键词：

D O I：

10.1038/NMETH1072

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Caged compounds are light- sensitive probes that functionally encapsulate biomolecules in an inactive form. Irradiation liberates the trapped molecule, permitting targeted perturbation of a biological process. Uncaging technology and fluorescence microscopy are ` optically orthogonal': the former allows control, and the latter, observation of cellular function. Used in conjunction with other technologies ( for example, patch clamp and/ or genetics), the light beam becomes a uniquely powerful tool to stimulate a selected biological target in space or time. Here I describe important examples of widely used caged compounds, their design features and synthesis, as well as practical details of how to use them with living cells.

引用

页码：619 / 628

页数：10

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