Caged compounds: photorelease technology for control of cellular chemistry and physiology

被引：731

作者：

Ellis-Davies, Graham C. R. ^{[1
]}

机构：

[1] Drexel Univ, Coll Med, Dept Physiol & Pharmacol, Philadelphia, PA 19102 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 08期

关键词：

D O I：

10.1038/NMETH1072

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Caged compounds are light- sensitive probes that functionally encapsulate biomolecules in an inactive form. Irradiation liberates the trapped molecule, permitting targeted perturbation of a biological process. Uncaging technology and fluorescence microscopy are ` optically orthogonal': the former allows control, and the latter, observation of cellular function. Used in conjunction with other technologies ( for example, patch clamp and/ or genetics), the light beam becomes a uniquely powerful tool to stimulate a selected biological target in space or time. Here I describe important examples of widely used caged compounds, their design features and synthesis, as well as practical details of how to use them with living cells.

引用

页码：619 / 628

页数：10

共 100 条

[31] Brominated 7-hydroxycoumarin-4-ylmethyls: Photolabile protecting groups with biologically useful cross-sections for two photon photolysis [J].

Furuta, T ;

Wang, SSH ;

Dantzker, JL ;

Dore, TM ;

Bybee, WJ ;

Callaway, EM ;

Denk, W ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (04) :1193-1200

[32] Photochemical reaction mechanisms of 2-nitrobenzyl compounds:: 2-Nitrobenzyl alcohols form 2-nitroso hydrates by dual proton transfer [J].

Gaplovsky, M ;

Il'ichev, YV ;

Kamdzhilov, Y ;

Kombarova, SV ;

Mac, M ;

Schwörer, MA ;

Wirz, J .

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2005, 4 (01) :33-42

[33] SYNTHESIS AND PHOTOCHEMISTRY OF A NEW PHOTOLABILE DERIVATIVE OF GABA - NEUROTRANSMITTER RELEASE AND RECEPTOR ACTIVATION IN THE MICROSECOND TIME REGION [J].

GEE, KR ;

WIEBOLDT, R ;

HESS, GP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (18) :8366-8367

[34] P3-[2-(4-hydroxyphenyl)-2-oxo]ethyl ATP for the rapid activation of the Na+,K+-ATPase [J].

Geibel, S ;

Barth, A ;

Amslinger, S ;

Jung, AH ;

Burzik, C ;

Clarke, RJ ;

Givens, RS ;

Fendler, K .

BIOPHYSICAL JOURNAL, 2000, 79 (03) :1346-1357

[35] Cofilin promotes actin polymerization and defines the direction of cell motility [J].

Ghosh, M ;

Song, XY ;

Mouneimne, G ;

Sidani, M ;

Lawrence, DS ;

Condeelis, JS .

SCIENCE, 2004, 304 (5671) :743-746

[36] Protein kinase C enhances exocytosis from chromaffin cells by increasing the size of the readily releasable pool of secretory granules [J].

Gillis, KD ;

Mossner, R ;

Neher, E .

NEURON, 1996, 16 (06) :1209-1220

[37]

Goeldner M, 2005, DYNAMIC STUDIES IN BIOLOGY: PHOTOTRIGGERS, PHOTOSWITCHES AND CAGED BIOMOLECULES, P1, DOI 10.1002/3527605592

[38] In vivo regulation of axon extension and pathfinding by growth-cone calcium transients [J].

Gomez, TM ;

Spitzer, NC .

NATURE, 1999, 397 (6717) :350-355

[39] Filopodial calcium transients promote substrate-dependent growth cone turning [J].

Gomez, TM ;

Robles, E ;

Poo, MM ;

Spitzer, NC .

SCIENCE, 2001, 291 (5510) :1983-1987

[40]

Hagen Volker, 2001, Angew Chem Int Ed Engl, V40, P1045, DOI 10.1002/1521-3773(20010316)40:6<1045::AID-ANIE10450>3.0.CO

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