Chromosomal transposition of PiggyBac in mouse embryonic stem cells

被引：296

作者：

Wang, Wei ^{[1
]}

Lin, Chengyi ^{[2
]}

Lu, Dong ^{[1
]}

Ning, Zeming ^{[1
]}

Cox, Tony ^{[1
]}

Melvin, David ^{[1
]}

Wang, Xiaozhong ^{[2
]}

Bradley, Allan ^{[1
]}

Liu, Pentao ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Hinxton CB10 1SA, Cambs, England

[2] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 27期

基金：

英国惠康基金;

关键词：

ES cell; Sleeping Beauty; transposon;

D O I：

10.1073/pnas.0801017105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transposon systems are widely used for generating mutations in various. model organisms. PiggyBac (PB) has recently been shown to transpose efficiently in the mouse germ line and other mammalian cell lines. To facilitate PB's application in mammalian genetics, we characterized the properties of the PB transposon in mouse embryonic stem (ES) cells. We first measured the transposition efficiencies of PB transposon in mouse embryonic stem cells. We next constructed a PB/SB hybrid transposon to compare PB and Sleeping Beauty (SB) transposon systems and demonstrated that PB transposition was inhibited by DNA methylation. The excision and reintegration rates of a single PB from two independent genomic loci were measured and its ability to mutate genes with gene trap cassettes was tested. We examined PB's integration site distribution in the mouse genome and found that PB transposition exhibited local hopping. The comprehensive information from this study should facilitate further exploration of the potential of PB and SB DNA transposons in mammalian genetics.

引用

页码：9290 / 9295

页数：6

共 37 条

[21] Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system [J].

Keng, VW ;

Yae, K ;

Hayakawa, T ;

Mizuno, S ;

Uno, Y ;

Yusa, K ;

Kokubu, C ;

Kinoshita, T ;

Akagi, K ;

Jenkins, NA ;

Copeland, NG ;

Horie, K ;

Takeda, J .

NATURE METHODS, 2005, 2 (10) :763-769

[22] Transposon-tagged mutagenesis in the rat [J].

Kitada, Kazuhiro ;

Ishishita, Satoshi ;

Tosaka, Keiko ;

Takahashi, Ri-ichi ;

Ueda, Masatsugu ;

Keng, Vincent W. ;

Horie, Kyoji ;

Takeda, Junji .

NATURE METHODS, 2007, 4 (02) :131-133

[23] Transposition of the piggyBac element in embryos of Drosophila melanogaster, Aedes aegypti and Trichoplusia ni [J].

Lobo, N ;

Li, X ;

Fraser, MJ .

MOLECULAR AND GENERAL GENETICS, 1999, 261 (4-5) :803-810

[24] Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells [J].

Luo, GB ;

Ivics, Z ;

Izsvák, Z ;

Bradley, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) :10769-10773

[25] THE ORIGIN AND BEHAVIOR OF MUTABLE LOCI IN MAIZE [J].

MCCLINTOCK, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1950, 36 (06) :344-355

[26] High-throughput retroviral tagging to identify components of specific signaling pathways in cancer (vol 32, pg 153, 2002) [J].

Mikkers, H ;

Allen, J ;

Knipscheer, P ;

Romeijn, L ;

Hart, A ;

Vink, E ;

Berns, A .

NATURE GENETICS, 2002, 32 (03) :459-459

[27] MOVERS AND SHAKERS - MAIZE TRANSPOSONS AS TOOLS FOR ANALYZING OTHER PLANT GENOMES [J].

OSBORNE, BI ;

BAKER, B .

CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (03) :406-413

[28] Sleeping Beauty transposition in the mouse genome is associated with changes in DNA methylation at the site of insertion [J].

Park, Chang Won ;

Park, Jeongmin ;

Kren, Betsy T. ;

Steer, Clifford J. .

GENOMICS, 2006, 88 (02) :204-213

[29] DNA methylation of Sleeping Beauty with transposition into the mouse genome [J].

Park, CW ;

Kren, BT ;

Largaespada, DA ;

Steer, CJ .

GENES TO CELLS, 2005, 10 (08) :763-776

[30]

Plasterk RHA, 1996, CURR TOP MICROBIOL, V204, P125

← 1 2 3 4 →