Chemical denaturants inhibit the onset of dewetting

被引：60

作者：

England, Jeremy L. ^{[2
]}

Pande, Vijay S. ^{[3
,4
]}

Haran, Gilad ^{[1
]}

机构：

[1] Weizmann Inst Sci, Dept Chem Phys, IL-76100 Rehovot, Israel

[2] Stanford Univ, Dept Phys, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Biol Struct, Stanford, CA 94305 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 36期

关键词：

D O I：

10.1021/ja803972g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The mechanism by which the aqueous cosolvents guanidinium chloride and urea denature proteins is a matter of controversy. Here, we use all-atom molecular dynamics simulations to study the effect of both denaturants on the dewetting of water confined between nanoseparated hydrophobic plates. It is found that the denaturants inhibit the onset of dewetting, so that it occurs at shorter interplate distances than in pure water. Our results support a role for urea and guanidinium in assisting in the solvation of nonpolar surfaces, thereby weakening hydrophobic effects known to be important for protein stability. Copyright © 2008 American Chemical Society.

引用

页码：11854 / 11855

页数：2

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