Optimization of In Vivo Confocal Autofluorescence Imaging of the Ocular Fundus in Mice and Its Application to Models of Human Retinal Degeneration

被引:51
作者
Issa, Peter Charbel [1 ,2 ]
Singh, Mandeep S. [1 ,2 ]
Lipinski, Daniel M. [1 ,2 ]
Chong, Ngaihang V. [1 ,2 ]
Delori, Francois C. [2 ,3 ,4 ]
Barnard, Alun R. [1 ]
MacLaren, Robert E. [1 ,2 ,5 ]
机构
[1] Univ Oxford, John Radcliffe Hosp, Nuffield Lab Ophthalmol, Oxford OX3 9DU, England
[2] Univ Oxford, John Radcliffe Hosp, Oxford Eye Hosp, Biomed Res Ctr, Oxford OX3 9DU, England
[3] Harvard Univ, Sch Med, Schepens Eye Res Inst, Boston, MA USA
[4] Harvard Univ, Sch Med, Dept Ophthalmol, Boston, MA USA
[5] Moorfields Eye Hosp, London, England
基金
英国惠康基金;
关键词
OPTICAL COHERENCE TOMOGRAPHY; SCANNING LASER OPHTHALMOSCOPY; PIGMENT EPITHELIUM; HIGH-RESOLUTION; RETINITIS-PIGMENTOSA; STARGARDTS-DISEASE; ANIMAL-MODELS; SCHEMATIC EYE; MOUSE; LIPOFUSCIN;
D O I
10.1167/iovs.11-8767
中图分类号
R77 [眼科学];
学科分类号
100212 [眼科学];
摘要
PURPOSE. To investigate the feasibility and to identify sources of experimental variability of quantitative and qualitative fundus autofluorescence (AF) assessment in mice. METHODS. Blue (488 nm) and near-infrared (790 nm) fundus AF imaging was performed in various mouse strains and disease models (129S2, C57Bl/6, Abca4(-/-), C3H-Pde6b(rd1/rd1), Rho(-/-), and BALB/c mice) using a commercially available scanning laser ophthalmoscope. Gray-level analysis was used to explore factors influencing fundus AF measurements. RESULTS. A contact lens avoided cataract development and resulted in consistent fundus AF recordings. Fundus illumination and magnification were sensitive to changes of the camera position. Standardized adjustment of the recorded confocal plane and consideration of the pupil area allowed reproducible recording of fundus AF from the retinal pigment epithelium with an intersession coefficient of repeatability of +/-22%. Photopigment bleaching occurred during the first 1.5 seconds of exposure to 488 nm blue light (similar to 10 mW/cm(2)), resulting in an increase of fundus AF. In addition, there was a slight decrease in fundus AF during prolonged blue light exposure. Fundus AF at 488 nm was low in animals with an absence of a normal visual cycle, and high in BALB/c and Abca4(-/-) mice. Degenerative alterations in Pde6b(rd1/rd1) and Rho(-/-) were reminiscent of findings in human retinal disease. CONCLUSIONS. Investigation of retinal phenotypes in mice is possible in vivo using standardized fundus AF imaging. Correlation with postmortem analysis is likely to lead to further understanding of human disease phenotypes and of retinal degenerations in general. Fundus AF imaging may be useful as an outcome measure in preclinical trials, such as for monitoring effects aimed at lowering lipofuscin accumulation in the retinal pigment epithelium. (Invest Ophthalmol Vis Sci. 2012;53:1066-1075) DOI:10.1167/iovs.11-8767
引用
收藏
页码:1066 / 1075
页数:10
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