Precise characterization of human histories in the H2A gene family by top down mass spectrometry

被引：132

作者：

Boyne, MT

Pesavento, JJ

Mizzen, CA

Kelleher, NL

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Cell & Dev Biol, Urbana, IL 61801 USA

[4] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA

[5] Univ Illinois, Ctr Top Down Proteom, Urbana, IL 61801 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 02期

关键词：

histone H2A; Fourier transform mass spectrometry (FTMS); Top Down; post-translational modification (PTM); SILAC; cell cycle; ubiquitination; electron capture dissociation (ECD); histone code; chromatin;

D O I：

10.1021/pr050269n

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species' revealed that all except H2A.Z and H2A.F/Z were alpha-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding similar to 10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.

引用

收藏

页码：248 / 253

页数：6

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