Development and validation of a method for the quantitation of Δ9 tetrahydrocannabinol in human plasma by high performance liquid chromatography after solid-phase extraction

被引：15

作者：

Abbara, Chadi

Galy, Romain

Benyamina, Amine

Reynaud, Michel

Bonhomme-Faivre, Laurence

机构：

[1] Hop Paul Brousse, AP HP, Pharmacol Lab, Serv Pharm, F-94800 Villejuif, France

[2] Hop Paul Brousse, AP HP, Serv Addictol, F-94800 Villejuif, France

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2006年 / 41卷 / 03期

关键词：

tetrahydrocannabinol; HPLC; analytical method validation;

D O I：

10.1016/j.jpba.2006.01.017

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high performance liquid chromatography (HPLC) procedure for the determination of Delta(9) tetrahydrocannabinol (THC) in human plasma is described. A two-step solid-phase extraction on CN cartridges was coupled with a reversed phase HPLC system. THC was eluted using a mobile phase composed of methanol, acetonitrile and tetrabutylammonium perchlorate solution (0.005 M, pH 3.2), through a C-18 Nucleosil column and detected at a wavelength of 215 nm. Calibration curve was linear over the range 5-100 ng/ml with a lower limit of quantification validated at 5 ng/ml. Extraction recovery using the developed extraction procedure was higher than 85%. This method is presently used for the quantification of THC in plasma samples from regular cannabis smokers. (c) 2006 Elsevier B.V. All rights reserved.

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收藏

页码：1011 / 1016

页数：6

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