Pronouced instability of tandem IU base pairs in RNA

被引:45
作者
Serra, MJ
Smolter, PE
Westhof, E
机构
[1] Allegheny Coll, Dept Chem, Meadville, PA 16335 USA
[2] CNRS, Inst Biol Mol & Cellulaire, UPR 9002, F-67084 Strasbourg, France
基金
美国国家科学基金会;
关键词
D O I
10.1093/nar/gkh501
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Optical melting was used to determine the stabilities of three series of RNA oligomers containing tandem XU base pairs, GGCXUGCC (5'XU3'), GGCUXGCC (5'UX3') and GGCXXGGC/CCGUUCCG (5'XX3'), where X is either A, G or I (inosine). The helices containing tandem AU base pairs were the most stable in the first two series (5'XU3' and 5'UX3'), with an average melting temperature similar to11degreesC higher than the helices with tandem 5'GU3' base pairs and 25degreesC higher than the helices with tandem 5'IU3' base pairs. For the third series (5'XX3'), the helix containing tandem GG is the most stable, with an average melting temperature similar to2degreesC higher than the helix with tandem AA base pairs and similar to24degreesC higher than the helix with tandem II base pairs. The thermodynamic stability of the oligomers with tandem IU base pairs was also investigated as a function of magnesium ion concentration. As with normal A-U or G-U tandem duplexes, the data could best be interpreted as non-specific binding of magnesium ions to the inosine-containing RNA oligonucleotides.
引用
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页码:1824 / 1828
页数:5
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