Metabolite profiling as an aid to metabolic engineering in plants

被引:105
作者
Trethewey, RN [1 ]
机构
[1] Metanom GmbH & Co KGaA, D-10589 Berlin, Germany
关键词
D O I
10.1016/j.pbi.2003.12.003
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The past decade has seen some impressive successes in the metabolic engineering of biotechnologically important plant pathways. However, plant metabolic engineering currently proceeds more by trial and error than by intelligent system design. A change in philosophy away from studying pathways in isolation and towards studying metabolism as a network is necessary. To support this development, improvements in technologies. for metabolic analysis, a wider adoption of metabolite-profiling approaches and significant innovations in data analysis methodologies are required.
引用
收藏
页码:196 / 201
页数:6
相关论文
共 62 条
[41]   'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data [J].
Nicholson, JK ;
Lindon, JC ;
Holmes, E .
XENOBIOTICA, 1999, 29 (11) :1181-1189
[42]   Metabonomics: a platform for studying drug toxicity and gene function [J].
Nicholson, JK ;
Connelly, J ;
Lindon, JC ;
Holmes, E .
NATURE REVIEWS DRUG DISCOVERY, 2002, 1 (02) :153-161
[43]   Starch content and yield increase as a result of altering adenylate pools in transgenic plants [J].
Regierer, B ;
Fernie, AR ;
Springer, F ;
Perez-Melis, A ;
Leisse, A ;
Koehl, K ;
Willmitzer, L ;
Geigenberger, P ;
Kossmann, J .
NATURE BIOTECHNOLOGY, 2002, 20 (12) :1256-1260
[44]   Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems [J].
Roessner, U ;
Luedemann, A ;
Brust, D ;
Fiehn, O ;
Linke, T ;
Willmitzer, L ;
Fernie, AR .
PLANT CELL, 2001, 13 (01) :11-29
[45]   Metabolic profiling and biochemical phenotyping of plant systems [J].
Roessner, U ;
Willmitzer, L ;
Fernie, AR .
PLANT CELL REPORTS, 2002, 21 (03) :189-196
[46]  
Roessner U, 2001, PLANT PHYSIOL, V127, P749, DOI [10.1104/pp.010316, 10.1104/pp.127.3.749]
[47]   Simultaneous analysis of metabolites in potato tuber by gas chromatography-mass spectrometry [J].
Roessner, U ;
Wagner, C ;
Kopka, J ;
Trethewey, RN ;
Willmitzer, L .
PLANT JOURNAL, 2000, 23 (01) :131-142
[48]   Metabolic profiling of transgenic tomato plants overexpressing hexokinase reveals that the influence of hexose phosphorylation diminishes during fruit development [J].
Roessner-Tunali, U ;
Hegemann, B ;
Lytovchenko, A ;
Carrari, F ;
Bruedigam, C ;
Granot, D ;
Fernie, AR .
PLANT PHYSIOLOGY, 2003, 133 (01) :84-99
[49]   Genetic engineering of a zeaxanthin-rich potato by antisense inactivation and co-suppression of carotenoid epoxidation [J].
Römer, S ;
Lübeck, J ;
Kauder, F ;
Steiger, S ;
Adomat, C ;
Sandmann, G .
METABOLIC ENGINEERING, 2002, 4 (04) :263-272
[50]   Metabolic engineering of osmoprotectant accumulation in plants [J].
Rontein, D ;
Basset, G ;
Hanson, AD .
METABOLIC ENGINEERING, 2002, 4 (01) :49-56