Electrochemical study of human olfactory receptor OR 17-40 stimulation by odorants in solution

被引：22

作者：

Benilova, I. V. ^{[2
,4
]}

Vidic, J. Minic ^{[3
]}

Pajot-Augy, E. ^{[3
]}

Soldatkin, A. P. ^{[4
]}

Martelet, C. ^{[2
]}

Jafftezic-Renault, N. ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, Sci Analyt Lab, UMR 5180, Bat Jules Raulin,5eme Etage, F-69622 Villeurbanne, France

[2] Ecole Cent Lyon, Lab AMPERE, UMR CNRS 5005, F-69134 Ecully, France

[3] INRA, Ctr Rech Jouy En Josas, UMR NOPA Neurobiol Olfact Prise Alimentaire 1197, F-78352 Jouy En Josas, France

[4] Inst Mol Biol & Genet NASU, Lab Biomol Elect, NASU, UA-03143 Kiev, Ukraine

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2008年 / 28卷 / 5-6期

关键词：

impedance spectroscopy; SPR; bioelectronic nose; human olfactory receptor; helional;

D O I：

10.1016/j.msec.2007.10.040

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The human olfactory receptor OR 17-40 co-expressed with alpha-subunit of G(olf) protein in yeast was attempted as a bio recognition part of impedimetric biosensor. The receptor in its natural membrane environment was anchored to a gold-coated glass substrate modified with thiol-based multilayer. Step-by-step building-up of the OR 17-40 based biofilm was monitored using surface plasmon resonance technique. Stimulation of the OR 17-40 with its cognate odorant helional in phosphate-buffered saline was probed by means of electrochemical impedance spectroscopy under various conditions. Activation of OR 17-40 in the presence of GTP-gamma-S at 4 degrees C was found to improve the sensitivity of the developed label-free biosensor, probably via the enhancement of the specific biochemical signal. (c) 2008 Elsevier B.V. All fights reserved.

引用

页码：633 / 639

页数：7

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