Oxidative biotransformations using oxygenases

被引：127

作者：

Li, Z ^{[1
]}

van Beilen, JB ^{[1
]}

Duetz, WA ^{[1
]}

Schmid, A ^{[1
]}

de Raadt, A ^{[1
]}

Griengl, H ^{[1
]}

Witholt, B ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, Inst Biotechnol, CH-8093 Zurich, Switzerland

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2002年 / 6卷 / 02期

关键词：

D O I：

10.1016/S1367-5931(02)00296-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Considerable progress has been made in manipulating oxidative biotransformations using oxygenases. Substrate acceptance, catalytic activity, regioselectivity and stereoselectivity have been improved significantly by substrate engineering, enzyme engineering or biocatalyst screening. Preparative biotransformations have been carried out to synthesize useful pharmaceutical intermediates or chiral synthons on the gram to several-hundred-gram scale, by use of whole cells of wild type or recombinant strains. The synthetic application of oxygenases in vitro has been shown to be possible by enzymatic or electrochemical regeneration of NADH or NADPH.

引用

页码：136 / 144

页数：9

共 60 条

[1] Biocatalytic asymmetric hydroxylation of hydrocarbons by free and immobilized Bacillus megaterium cells [J].

Adam, W ;

Lukacs, Z ;

Kahle, C ;

Saha-Möller, CR ;

Schreier, P .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 11 (4-6) :377-385

[2] Biotransformations by Botrytis species [J].

Aleu, J ;

Collado, IG .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2001, 13 (4-6) :77-93

[3] A P450BM-3 mutant hydroxylates alkanes, cycloalkanes, arenes and heteroarenes [J].

Appel, D ;

Lutz-Wahl, S ;

Fischer, P ;

Schwaneberg, U ;

Schmid, RD .

JOURNAL OF BIOTECHNOLOGY, 2001, 88 (02) :167-171

[4] A new example of 1α-hydroxylation of drimanic terpenes through combined microbial and chemical processes [J].

Aranda, G ;

Moreno, L ;

Cortés, M ;

Prangé, T ;

Maurs, M ;

Azerad, R .

TETRAHEDRON, 2001, 57 (28) :6051-6056

[5] Production of cyclohexanone monooxygenase from Acinetobacter calcoaceticus for large scale Baeyer-Villiger monooxygenase reactions [J].

Barclay, SS ;

Woodley, JM ;

Lilly, MD ;

Spargo, PL ;

Pettman, AJ .

BIOTECHNOLOGY LETTERS, 2001, 23 (05) :385-388

[6] Enantiopure arene dioxides:: chemoenzymatic synthesis and application in the production of trans-3,4-dihydrodiols [J].

Boyd, DR ;

Sharma, ND ;

O'Dowd, CR ;

Hempenstall, F .

CHEMICAL COMMUNICATIONS, 2000, (21) :2151-2152

[7] Biotransformation of diterpenes and diterpene derivatives by Beauveria bassiana ATCC 7159 [J].

Buchanan, GO ;

Reese, PB .

PHYTOCHEMISTRY, 2001, 56 (02) :141-151

[8] Protein engineering of Bacillus megaterium CYP102 -: The oxidation of polycyclic aromatic hydrocarbons [J].

Carmichael, AB ;

Wong, LL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (10) :3117-3125

[9] The use of oxygen uptake rate measurements to control the supply of toxic substrate:: toluene hydroxylation by Pseudomonas putida UV4 [J].

Carragher, JM ;

McClean, WS ;

Woodley, JM ;

Hack, CJ .

ENZYME AND MICROBIAL TECHNOLOGY, 2001, 28 (2-3) :183-188

[10] Preparation of (S)-N-substituted 4-hydroxy-pyrrolidin-2-ones by regio-and stereoselective hydroxylation with Sphingomonas sp HXN-200 [J].

Chang, DL ;

Witholt, B ;

Li, Z .

ORGANIC LETTERS, 2000, 2 (24) :3949-3952

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