Cross-linked crystals of subtilisin: Versatile catalyst for organic synthesis

被引：53

作者：

Wang, YF

Yakovlevsky, K

Zhang, BL

Margolin, AL

机构：

[1] Altus Biologics Inc., Cambridge, MA 02139-4211

来源：

JOURNAL OF ORGANIC CHEMISTRY | 1997年 / 62卷 / 11期

关键词：

D O I：

10.1021/jo9618334

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Cross-linked enzyme crystals (CLECs) of subtilisin exhibit excellent activity in aqueous and various organic solvents. This catalyst is more stable than the native enzyme in both aqueous and mixed aqueous/organic solutions. Subtilisin-CLEC was shown to be a versatile catalyst. It was used for the syntheses of peptides and peptidomimetics, mild hydrolysis of amino acid and peptide amides, enantio- and regioselective reactions, and transesterifications.

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页码：3488 / 3495

页数：8

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