A cDNA-AFLP approach to study ochratoxin A production in Aspergillus carbonarius

被引：16

作者：

Botton, Alessandro ^{[1
]}

Ferrigo, Davide ^{[2
]}

Scopel, Cristina ^{[2
]}

Causin, Roberto ^{[2
]}

Bonghi, Claudio ^{[1
]}

Ramina, Angelo ^{[1
]}

机构：

[1] Univ Padua, Dept Environm Agron & Crop Sci, I-35020 Padua, Italy

[2] Univ Padua, Dip Terr & Sistemi Agroforestali, TESAF, I-35020 Padua, Italy

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2008年 / 127卷 / 1-2期

关键词：

Aspergillus carbonarius; ochratoxin A biosynthesis; cDNA-AFLP differential display; G protein; Ca(2+)/calmodulin dependent phosphorylation and dephoshorylation; zinc finger transcription factors;

D O I：

10.1016/j.ijfoodmicro.2008.06.037

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Aspergillus carbonarius is responsible for the majority of mycotoxin contaminations in grapes and its derivatives. Most of A. carbonarius strains are ochratoxin A (OTA) producers, even though at very different levels. This broad variability was used to identify genes whose expression is linked with the ability of producing OTA. A cDNA-AFLP differential display screening was performed in two strains of A. carbonarius, antagonists for the ability of producing OTA, allowing the identification of 119 differentially expressed sequences putatively involved in the regulation of OTA biosynthesis. A likely connection was pointed out between the biosynthesis of the toxin, vegetative growth and sexual/asexual developmental progression, along with common signalling pathways involving G protein and Ca(2+)/calmodulin dependent phosphorylation and dephoshorylation cascades. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：105 / 115

页数：11

共 77 条

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