Microfabrication Technology for the Production of Capillary Array Electrophoresis Chips

被引:110
作者
Simpson, Peter C. [1 ]
Woolley, Adam T. [1 ]
Mathies, Richard A. [2 ]
机构
[1] Univ Calif Berkeley, Dept Chem, Mathies Res Grp, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
基金
美国国家卫生研究院;
关键词
DNA Analysis; capillary array electrophoresis; confocal fluorescence detection; multiplex sample injection; glass micro-machining; capillary electrophoresis chips;
D O I
10.1023/A:1009922004301
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Improvements in the fabrication, sample handling and electrical addressing of capillary array electrophoresis (CAE) chips have permitted the development of high density, high-throughput devices capable of analyzing 48 samples in about 20 minutes. The fabrication of high density capillary arrays on 10 cm diameter substrates required the characterization of glasses that yield high quality etches and the development of improved sacrificial etch masks. Using these improved fabrication techniques, high-quality, deep channel etches are routinely obtained. Methods for bonding large area substrates and for drilling arrays of 100 or more access holes have also been developed. For easier sample introduction, we use an array of sample wells fabricated from an elastomeric sheet. The practicality of these technologies is demonstrated through the analysis of 12 DNA samples in parallel on a microfabricated CAE chip, the development of methods for injecting multiple samples onto a single capillary without cross contamination, and the operation of a microfabricated array of 12 capillaries with 4 sample injections per capillary that can analyze 48 samples.
引用
收藏
页码:7 / 25
页数:19
相关论文
共 26 条
  • [1] RAPID DNA SEQUENCING BY HORIZONTAL ULTRATHIN GEL-ELECTROPHORESIS
    BRUMLEY, RL
    SMITH, LM
    [J]. NUCLEIC ACIDS RESEARCH, 1991, 19 (15) : 4121 - 4126
  • [2] Microfabricated structures for integrated DNA analysis
    Burns, MA
    Mastrangelo, CH
    Sammarco, TS
    Man, FP
    Webster, JR
    Johnson, BN
    Foerster, B
    Jones, D
    Fields, Y
    Kaiser, AR
    Burke, DT
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (11) : 5556 - 5561
  • [3] FABRICATION OF IN-VITRO MICROVASCULAR BLOOD-FLOW SYSTEMS BY PHOTOLITHOGRAPHY
    COKELET, GR
    SOAVE, R
    PUGH, G
    RATHBUN, L
    [J]. MICROVASCULAR RESEARCH, 1993, 46 (03) : 394 - 400
  • [4] HIGH-SPEED SEPARATION OF ANTISENSE OLIGONUCLEOTIDES ON A MICROMACHINED CAPILLARY ELECTROPHORESIS DEVICE
    EFFENHAUSER, CS
    PAULUS, A
    MANZ, A
    WIDMER, HM
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (18) : 2949 - 2953
  • [5] GLASS CHIPS FOR HIGH-SPEED CAPILLARY ELECTROPHORESIS SEPARATIONS WITH SUBMICROMETER PLATE HEIGHTS
    EFFENHAUSER, CS
    MANZ, A
    WIDMER, HM
    [J]. ANALYTICAL CHEMISTRY, 1993, 65 (19) : 2637 - 2642
  • [6] MICROMACHINING OF CAPILLARY ELECTROPHORESIS INJECTORS AND SEPARATORS ON GLASS CHIPS AND EVALUATION OF FLOW AT CAPILLARY INTERSECTIONS
    FAN, ZH
    HARRISON, DJ
    [J]. ANALYTICAL CHEMISTRY, 1994, 66 (01) : 177 - 184
  • [7] Integrated capillary electrophoresis devices with an efficient postcolumn reactor in planar quartz and glass chips
    Fluri, K
    Fitzpatrick, G
    Chiem, N
    Harrison, DJ
    [J]. ANALYTICAL CHEMISTRY, 1996, 68 (23) : 4285 - 4290
  • [8] MICROMACHINING A MINIATURIZED CAPILLARY ELECTROPHORESIS-BASED CHEMICAL-ANALYSIS SYSTEM ON A CHIP
    HARRISON, DJ
    FLURI, K
    SEILER, K
    FAN, ZH
    EFFENHAUSER, CS
    MANZ, A
    [J]. SCIENCE, 1993, 261 (5123) : 895 - 897
  • [9] CAPILLARY ELECTROPHORESIS AND SAMPLE INJECTION SYSTEMS INTEGRATED ON A PLANAR GLASS CHIP
    HARRISON, DJ
    MANZ, A
    FAN, ZH
    LUDI, H
    WIDMER, HM
    [J]. ANALYTICAL CHEMISTRY, 1992, 64 (17) : 1926 - 1932
  • [10] HIGH-PERFORMANCE ELECTROPHORESIS - ELIMINATION OF ELECTROENDOSMOSIS AND SOLUTE ADSORPTION
    HJERTEN, S
    [J]. JOURNAL OF CHROMATOGRAPHY, 1985, 347 (02): : 191 - 198