Composite electrochemical biosensors:: a comparison of three different electrode matrices for the construction of amperometric tyrosinase biosensors

被引：71

作者：

Serra, B ^{[1
]}

Jiménez, S ^{[1
]}

Mena, ML ^{[1
]}

Reviejo, AJ ^{[1
]}

Pingarrón, JM ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Chem, Dept Analyt Chem, Madrid 28040, Spain

来源：

BIOSENSORS & BIOELECTRONICS | 2002年 / 17卷 / 03期

关键词：

composite electrode matrices; tyrosinase biosensors; phenolic compounds;

D O I：

10.1016/S0956-5663(01)00269-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A comparison of the behaviour of three different rigid composite matrices for the construction of amperometric tyrosinase biosensors. which are widely used for the detection of phenolic compounds. is reported. The composite electrode matrices were, graphite-Teflon; reticulated vitreous carbon (RVC)-epoxy resin; and graphite-ethylene/propylene/diene (EPD) terpolymer. After optimization of the experimental conditions, different aspects regarding the stability of the three composite tyrosinase electrode designs were considered and compared. A better reproducibility of the amperometric responses was found with the graphite-EPD electrodes, whereas a longer useful lifetime was observed for the graphite-Teflon electrodes. The kinetic parameters of the tyrosinase reaction were calculated for eight different phenolic compounds, as well as their corresponding calibration plots. The general trend in sensitivity was graphite-EPD > graphite-Teflon much greater than RVC-epoxy resin. A correlation between sensitivity and the catalytic efficiency of the enzyme reaction for each phenolic substrate was found. Furthermore, differences in the sensitivity order for the phenolic compounds were observed among the three biocomposite electrodes. which suggests that the nature of the electrode matrix influences the interactions in the tyrosinase catalytic cycle. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：217 / 226

页数：10

共 29 条

[1]

Alegret S, 1996, SENSOR MATER, V8, P147

[2]

Alonso L, 1999, ELECTROANAL, V11, P161

[3] BIOCHEMICAL ASPECTS OF BIOSENSORS [J].

BYFIELD, MP ;

ABUKNESHA, RA .

BIOSENSORS & BIOELECTRONICS, 1994, 9 (4-5) :373-400

[4] Development of a bienzymic graphite-Teflon composite electrode for the determination of hypoxanthine in fish [J].

Cayuela, G ;

Peña, N ;

Reviejo, AJ ;

Pingarrón, JM .

ANALYST, 1998, 123 (02) :371-377

[5] New materials for electrochemical sensing .1. Rigid conducting composites [J].

Cespedes, F ;

MartinezFabregas, E ;

Alegret, S .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1996, 15 (07) :296-304

[6] Enhancement of biosensor sensitivity in aqueous and organic solvents using a combination of poly(pyrrole-ammonium) and poly(pyrrole-lactobionamide) films as host matrices. [J].

Cosnier, S ;

Lepellec, A ;

Guidetti, B ;

Rico-Lattes, I .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1998, 449 (1-2) :165-171

[7] Reagentless tyrosinase enzyme electrodes: Effects of enzyme loading, electrolyte pH, ionic strength, and temperature [J].

Daigle, F ;

Leech, D .

ANALYTICAL CHEMISTRY, 1997, 69 (20) :4108-4112

[8] Graphite-teflon-peroxidase composite electrodes. Application to the direct determination of glucose in musts and wines [J].

del Cerro, MA ;

Cayuela, G ;

Reviejo, AJ ;

Pingarron, JM ;

Wang, J .

ELECTROANALYSIS, 1997, 9 (14) :1113-1119

[9]

Ducey MW, 1998, ELECTROANAL, V10, P157, DOI 10.1002/(SICI)1521-4109(199803)10:3<157::AID-ELAN157>3.0.CO

[10]

2-O

← 1 2 3 →