Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae

被引：12

作者：

Daniel, JA

Yoo, JY

Bettinger, BT

Amberg, DC

Burke, DJ

机构：

[1] Univ Virginia, Med Ctr, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA

[2] SUNY Upstate Med Univ, Dept Biochem & Mol Biol, Syracuse, NY 13210 USA

来源：

GENETICS | 2006年 / 172卷 / 01期

关键词：

D O I：

10.1534/genetics.105.047662

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3 Delta 1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5(+) gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.

引用

页码：709 / 711

页数：3

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