Active capture and transport of virus particles using a biomolecular motor-driven, nanoscale antibody sandwich assay

被引：95

作者：

Bachand, GD

Rivera, SB

Carroll-Portillo, A

Hess, H

Bachand, M

机构：

[1] Sandia Natl Labs, Biomol Mat & Interfaces Dept, Albuquerque, NM 87185 USA

[2] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

[3] Univ Florida, Dept Mat Sci & Engn, Gainesville, FL 32611 USA

来源：

SMALL | 2006年 / 2卷 / 03期

关键词：

biomotecular motors; kinesin; nanodevices; nanofluidics; transport;

D O I：

10.1002/smll.200500262

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The technique of active capture and transport of virus particles using a biomolecular motor-driven, nanoscale antibody sandwich assay was described. Nanofluidic transport of macromolecules within living cells is achieved using a complex, three-dimensional network of cytoskeletal filaments and motor proteins. It is observed that glutaraldehyde crosslinking successfully linked fluorescent antibodies to MT shuttles. The application of kinesin and Ab-MT as mechanical actuators enables the development of nanofluidic systems that rely only on chemical energy for capturing and separating of target analytes from a complex solution.

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收藏

页码：381 / 385

页数：5

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